thiT

S protein of thiamine ECF transporter

Locus: BSU_30990

Isoelectric point: 10.24

Molecular weight: 20.31 kDa

Protein length: 192 aa

Gene length: 579 bp

Function: thiamine uptake

Product: S protein of thiamine ECF transporter

Essential: no

Synonyms: yuaJ

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ECF transporter
• Cellular processes → Transporters → ECF transporter → The substrate-specific S components of the ECF transporters
• Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis/ acquisition of thiamine
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,179,306 3,179,884

The protein

Protein family

• vitamin uptake transporter (VUT/ECF) (TC 2.A.88) family (with TrpP and YpdP, according to UniProt)

Structure

• 3RLB (from Lactococcus lactis, 32% identity) PubMed

Localization

• membrane PubMed

Expression and Regulation

Operons

• Genes: thiT
  Description: PubMed

  Regulatory mechanism

  • Thi-box: RNA switch, in Thi-box regulon

  Regulation

  • repressed by thiamine (Thi-box) PubMed

Biological materials

Mutant

• MGNA-A222 (yuaJ::erm), available at the NBRP B. subtilis, Japan
• BKE30990 (thiT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATCTATCTCCTTCCATT, downstream forward: _UP4_TAAAAGTAACAATCCCCCAG
• BKK30990 (thiT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATCTATCTCCTTCCATT, downstream forward: _UP4_TAAAAGTAACAATCCCCCAG

References

Reviews

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Original publications

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