yhfW

similar to Rieske 2Fe-2S iron-sulfur protein

locus: BSU_10390

pI: 5.66

mw: 56.94 kDa

protein length: 509 aa

gene length: 1530 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.4|Electron transport/ other/ based on similarity]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

Gene

Coordinates: 1,114,057 1,115,586

Phenotypes of a mutant

• reduced transfomation efficiency [pubmed|32249531]
• reduced amounts of citric acid cycle intermediates [pubmed|32249531]

The protein

[SW|Domains]

• Rieske domain (aa 423-509) (according to UniProt)

[SW|Cofactors]

• Fe-S cluster [pubmed|29292548]

Structure

• [PDB|4P9S] (dimethylglycine dehydrogenase from Rattus norvegicus, corresponds to aa 22 ... 396, 25% identity) [pubmed|24858690]

Expression and Regulation

Operons

• genes: [gene|96136B1EDFAA1A34ED012CB5BA1DDFB8A95FB4A1|yhfW]
  description: [Pubmed|15033535]

  sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|15699190], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

  regulation

  • expressed during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]) [Pubmed|15699190]
  • highly upregulated in competent cells as compared to non-competent cells [pubmed|32249531]

Biological materials

Mutant

• MGNA-A716 (yhfW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/716 NBRP B. subtilis, Japan]
• BKE10390 ([gene|96136B1EDFAA1A34ED012CB5BA1DDFB8A95FB4A1|yhfW]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGCCGTTATACCTCCAAT, downstream forward: _UP4_TAAACCGTTCATTTTAATGA
• BKK10390 ([gene|96136B1EDFAA1A34ED012CB5BA1DDFB8A95FB4A1|yhfW]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGCCGTTATACCTCCAAT, downstream forward: _UP4_TAAACCGTTCATTTTAATGA

References

• 16497325,15033535,15699190,32249531,24858690