yhfW

similar to Rieske 2Fe-2S iron-sulfur protein

Locus: BSU_10390

Isoelectric point: 5.66

Molecular weight: 56.94 kDa

Protein length: 509 aa

Gene length: 1530 bp

Function: unknown

Product: unknown

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Electron transport and ATP synthesis → Electron transport/ other/ based on similarity
• Information processing → Genetics → Genetic competence
• Lifestyles → Exponential and early post-exponential lifestyles → Genetic competence
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

Gene

Coordinates: 1,114,057 1,115,586

Phenotypes of a mutant

• reduced transfomation efficiency PubMed
• reduced amounts of citric acid cycle intermediates PubMed

The protein

Domains

• Rieske domain (aa 423-509) (according to UniProt)

Cofactors

• Fe-S cluster PubMed

Structure

• 4P9S (dimethylglycine dehydrogenase from Rattus norvegicus, corresponds to aa 22 ... 396, 25% identity) PubMed

Expression and Regulation

Operons

• Genes: yhfW
  Description: PubMed

  Sigma factors

  • SigF: sigma factor, PubMed, in SigF regulon

  Regulation

  • expressed during sporulation in the forespore (SigF) PubMed
  • highly upregulated in competent cells as compared to non-competent cells PubMed

Biological materials

Mutant

• MGNA-A716 (yhfW::erm), available at the NBRP B. subtilis, Japan
• BKE10390 (yhfW::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGCCGTTATACCTCCAAT, downstream forward: _UP4_TAAACCGTTCATTTTAATGA
• BKK10390 (yhfW::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGCCGTTATACCTCCAAT, downstream forward: _UP4_TAAACCGTTCATTTTAATGA

References

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