yaaI

general stress protein, similar to isochorismatase

locus: BSU_00170

pI: 4.67

mw: 20.66 kDa

protein length: 181 aa

gene length: 546 bp

function: survival of ethanol stress

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 25,221 25,766

The protein

Protein family

• [SW|Isochorismatase family] (according to UniProt)

Structure

• [PDB|3TG2] (from Vibrio cholerae, 26% identity) [pubmed|22993087]

Additional information

• important for survival of ethanol stress [Pubmed|15805528]

Expression and Regulation

Operons

• genes: [gene|95C78F511C49E04241D31593E6837BDFF48D6E14|yaaI]-[gene|77BEC74867422AA225A1F12AD63464656DBDCBE8|yaaH]
  description: [pubmed|22383849]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulation

  • induced by stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]) [Pubmed|15805528]

Biological materials

Mutant

• MGNA-B890 (yaaI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1889 NBRP B. subtilis, Japan]
• BKE00170 ([gene|95C78F511C49E04241D31593E6837BDFF48D6E14|yaaI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCGACAGTCTCCTTTCC, downstream forward: _UP4_TAAACATGATCAGCGCTTTT
• BKK00170 ([gene|95C78F511C49E04241D31593E6837BDFF48D6E14|yaaI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCGACAGTCTCCTTTCC, downstream forward: _UP4_TAAACATGATCAGCGCTTTT

References

• 15805528,15805528,22993087