dhbF [2018-02-11T17:00:06.000Z]

involved in 2,3-dihydroxybenzoate biosynthesis

locus: BSU31960

pI: 4.74

mw: 263.44 kDa

protein length: 2378 aa

gene length: 7134 bp

function: biosynthesis of the siderophore bacillibactin

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.3|Acquisition of iron / Other]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.1|Acquisition of iron / Other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

Gene

Coordinates: 3,280,519 → 3,287,655

The protein

Protein family

• ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

Paralogous protein(s)

• shares highly conserved domains of more than 1,000 amino acids with: [protein|95B75CFF126AF0AD845E62B4036DD3B6DAC8C7FA|DhbF], [protein|13EB6B903088C11D985DDB7CEC864797058FE038|PksJ], [protein|66AFF1FC7D1BBA8EA5BB57A935FF5AB890A895C1|PksN], [protein|E3D7EF8F165CAC2682B0D620081C5C6E555E43C4|PpsA], [protein|E7A2F93B33B1899313893D362D92405232BC9097|PpsB], [protein|D11CE89E2AC2ADE3FEFEC261E295EC5906CE6407|PpsC], [protein|E73D9F1A8F54DAD4B2056CABBE580D7AD36CB042|PpsD], [protein|C1C051209943E02369356075EAA733297E9478CB|PpsE], [protein|81845F44CE2C601555066E31E87384FF5D7B4139|SrfAA], [protein|3C484FDB3A440269E87D6B6E8500A875AC456666|SrfAB], [protein|1D5F227860A321506A1AB4BAE63CAD3A66E43BFC|SrfAC]

Modification

• phosphorylation on Ser-988 AND Ser-996 [Pubmed|17218307]

Structure

• [PDB|2VSQ] (termination module of [protein|1D5F227860A321506A1AB4BAE63CAD3A66E43BFC|SrfAC], 31% identity) [pubmed|18583577]

[SW|Localization]

• membrane [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|42C8D303AABFA2A9706295A0F90B0B49F854B0BF|dhbA]-[gene|0D4D200095AD4F9B30179B9AF500306EDA61F5D1|dhbC]-[gene|B864EC6D21AD5E524AC13AE1BE41F5CD7FC399C5|dhbE]-[gene|1346D57F906EE5BD36F7FBFA1E4884EEBC8585FA|dhbB]-[gene|95B75CFF126AF0AD845E62B4036DD3B6DAC8C7FA|dhbF]-[gene|DD647083C2E6968AD26A41C9F4EFBA942D75207C|ybdZ]
  description: [Pubmed|8921902]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8550523], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI]: sigma factor, [pubmed|29914988], in [regulon|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI regulon]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|Kre]: repression, in [regulon|65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|Kre regulon]

  regulation

  • induced by iron starvation (second wave to allow iron scavenging from the environment) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]) [Pubmed|29133393,12354229]
  • expression of the operon is strongly induced at the beginning of [SW|biofilm formation] [pubmed|31113899]

  additional information

  • the ''[gene|42C8D303AABFA2A9706295A0F90B0B49F854B0BF|dhbA]-[gene|0D4D200095AD4F9B30179B9AF500306EDA61F5D1|dhbC]-[gene|B864EC6D21AD5E524AC13AE1BE41F5CD7FC399C5|dhbE]-[gene|1346D57F906EE5BD36F7FBFA1E4884EEBC8585FA|dhbB]-[gene|95B75CFF126AF0AD845E62B4036DD3B6DAC8C7FA|dhbF]'' operon is strongly unregulated in a ''[SW|kre]'' mutant [Pubmed|26110430]

Biological materials

Mutant

• MGNA-A063 (yukL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/63 NBRP B. subtilis, Japan]
• BKE31960 (Δ[gene|95B75CFF126AF0AD845E62B4036DD3B6DAC8C7FA|dhbF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGTTTGTTTACCCTCC, downstream forward: _UP4_AATAAATAAGGGAGGAGAAA
• BKK31960 (Δ[gene|95B75CFF126AF0AD845E62B4036DD3B6DAC8C7FA|dhbF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGTTTGTTTACCCTCC, downstream forward: _UP4_AATAAATAAGGGAGGAGAAA

References

• 11112781,11790741,12354229,18763711,17218307,8921902,8550523,20817675,21815947,24223955,26110430,18583577,29133393