pdhA

pyruvate dehydrogenase (E1 alpha subunit), required for Z-ring assembly in a pyruvate-dependent manner

locus: BSU_14580

pI: 5.84

mw: 41.39 kDa

protein length: 371 aa

gene length: 1116 bp

function: links glycolysis and TCA cycle

product: pyruvate dehydrogenase (E1 alpha subunit)

essential: no

ec: 1.2.4.1

synonyms: aceA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]

Gene

Coordinates: 1,528,326 1,529,441

Phenotypes of a mutant

• ''pdhA'' is essential according to Kobayashi ''et al''. [Pubmed|12682299], non-essential according to [Pubmed|28189581]
• the mutant grows slowly but is viable [Pubmed|24825009]
• depletion of ''[gene|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA]'' and deletion of ''[gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA]'' have a strong synthetic defect in [SW|cell division] [Pubmed|24825009]

The protein

Catalyzed reaction/ biological activity

• [dihydrolipoyllysine-residue acetyltransferase]-(R)-N⁶-lipoyl-L-lysine + H⁺ + pyruvate --> [dihydrolipoyllysine-residue acetyltransferase]-(R)-N⁶-(S⁸-acetyldihydrolipoyl)-L-lysine + CO₂ (according to UniProt)

Paralogous protein(s)

• [protein|9F298088C0A9EB7FE140C935AFC9243C6D4DE8AE|BkdAA], [protein|B0A333BDFAE816B48B856A1DD91B73C41584A8E4|AcoA]

Kinetic information

• Michaelis-Menten [Pubmed|6414463]

[SW|Cofactors]

• thiamine pyrophosphate

Effectors of protein activity

• Inhibited thiamine 2-thiothiazolone diphosphate and NADH [Pubmed|6414463]
• Low sensibility to NADPH

Structure

• [PDB|1W88] (E1 in complex with subunit binding domain of E2, ''Geobacillus stearothermophilus'')

[SW|Localization]

• colocalizes with the nucleoid (depending on the availability of pyruvate) [Pubmed|24825009]

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA]-[gene|458E967052D1093A0F48AE0E6B6CCA0F52EAC44D|pdhB]-[gene|2F40086E35FA32136B9A89C530A86D714FE9460C|pdhC]-[gene|E9BBAE86DF3E536A987179CC394B472F6F710498|pdhD]
  description: [Pubmed|11976308]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|20081037], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, due to presence of guanine at 1 position of the transcript [Pubmed|20081037], in [regulon|stringent response|stringent response]

  regulation

  • ''[protein|search|pdhA]'': expression activated by glucose (1.9-fold) [Pubmed|12850135]

additional information

• the mRNA belongs to the 46 most abundant mRNA species in dormant spores [pubmed|30782632]

Biological materials

Mutant

• BKE14580 ([gene|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTAAGTCACCTCTTCC, downstream forward: _UP4_ACACAGAAGGAGTCGAAGTA
• BKK14580 ([gene|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTAAGTCACCTCTTCC, downstream forward: _UP4_ACACAGAAGGAGTCGAAGTA

lacZ fusion

• pGP721 (in [SW|pAC5]), available in [SW|Jörg Stülke]'s lab, pGP186 (in [SW|pAC7]), available in [SW|Jörg Stülke]'s lab

labs

• [SW|Arthur Aronson], Purdue University, West Lafayette, USA [http://wwwdev.gradschool.purdue.edu/PULSe/faculty.cfm?fid=5&range=0 homepage]

References

Reviews

• 19476487,9655937,2227213,6805383,24798336

Original publications

• 9352926,20525796,12850135,6414463,11976308,20081037,15378759,24825009,28189581,28516784,30782632