spoVAD

essential for the uptake of the 1:1 chelate of pyridine-2,6-dicarboxylic acid (DPA(2,6)) and Ca(2 ) into developing spores, likely germination protein

Locus: BSU_23410

Isoelectric point: 5.24

Molecular weight: 35.84 kDa

Protein length: 338 aa

Gene length: 1017 bp

Function: likely germination protein

Product: binding protein for DPA

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Sporulation → Germination → Additional germination proteins
• Groups of genes → Membrane proteins

Gene

Coordinates: 2,440,775 2,441,791

The protein

Catalyzed reaction/ biological activity

• maybe involved in spore germination PubMed
• binding of both DPA(2,6) and Ca(2 )-DPA(2,6) PubMed

Structure

• 3LM6

Localization

• outer surface of the inner spore membrane PubMed

Additional information

• 6,500 molecules are present per spore PubMed

Expression and Regulation

Operons

• Genes: spoVAA-spoVAB-spoVAC-spoVAD-spoVAEB-spoVAEA-spoVAF-lysA
  Description: PubMed

  Sigma factors

  • SigG: sigma factor, PubMed, in SigG regulon

  Regulatory mechanism

  • SpoVT: activation, PubMed, in SpoVT regulon

  Regulation

  • expressed late during sporulation in the forespore (SigG, SpoVT) PubMed

Additional information

• 6,500 molecules are present per spore PubMed

Biological materials

Mutant

• BKE23410 (spoVAD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TGTTAATTTCATTTTCTTCT, downstream forward: _UP4_GAGCGTGCAGGAGGTGCATC
• BKK23410 (spoVAD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TGTTAATTTCATTTTCTTCT, downstream forward: _UP4_GAGCGTGCAGGAGGTGCATC

Labs working on this gene/protein

• Peter Setlow, University of Connecticut Health Center, USA

References

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