spoVAD

essential for the uptake of the 1:1 chelate of pyridine-2,6-dicarboxylic acid (DPA(2,6)) and Ca(2 ) into developing spores, likely germination protein

locus: BSU_23410

pI: 5.24

mw: 35.84 kDa

protein length: 338 aa

gene length: 1017 bp

function: likely germination protein

product: binding protein for DPA

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.4|Germination] → [category|SW 4.2.4.2|Additional germination proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,440,775 2,441,791

The protein

Catalyzed reaction/ biological activity

• maybe involved in spore [SW|germination] [Pubmed|22327596]
• binding of both DPA(2,6) and Ca(2 )-DPA(2,6) [Pubmed|22328679]

Structure

• [PDB|3LM6]

[SW|Localization]

• outer surface of the inner spore membrane [Pubmed|23335419,16077113]

Additional information

• 6,500 molecules are present per spore [Pubmed|23749970]

Expression and Regulation

Operons

• genes: [gene|5410018998EC9792A69CF0D9C438EEF5AC8A82C5|spoVAA]-[gene|AEC70413BDC29F435776D532E7049AA9A0BD893D|spoVAB]-[gene|B280951A232E941FB0A6C8834049765BE1E16437|spoVAC]-[gene|9531F0A0DC4AE5696FFBAC9C2A773F15840E3255|spoVAD]-[gene|31D6CB7EC20C39C1815CA4552B8B9C4BE304E86B|spoVAEB]-[gene|B44529B69769D7AF1010E06C95E398EA91A3E798|spoVAEA]-[gene|CBE5093DFBBCE25071CCE12408BC9AFF7A158C03|spoVAF]-[gene|02F1C926D200DE85C93A0ACEF23AD25FBD12B0BD|lysA]
  description: [Pubmed|7934830]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|15699190,1903432], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulatory mechanism

  • [protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|SpoVT]: activation, [Pubmed|8755877], in [regulon|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|SpoVT regulon]

  regulation

  • expressed late during sporulation in the forespore ([protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG], [SW|SpoVT]) [Pubmed|15699190,1903432,8755877]

additional information

• 6,500 molecules are present per spore [PubMed|23749970]

Biological materials

Mutant

• BKE23410 ([gene|9531F0A0DC4AE5696FFBAC9C2A773F15840E3255|spoVAD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTTAATTTCATTTTCTTCT, downstream forward: _UP4_GAGCGTGCAGGAGGTGCATC
• BKK23410 ([gene|9531F0A0DC4AE5696FFBAC9C2A773F15840E3255|spoVAD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTTAATTTCATTTTCTTCT, downstream forward: _UP4_GAGCGTGCAGGAGGTGCATC

labs

• [SW|Peter Setlow], University of Connecticut Health Center, USA

References

• 17573930,3114420,16077113,11751839,15451103,3926949,17158659,6432957,22328679,7934830,15699190,1903432,8755877,22327596,22328679,22493018,23335419,23746146,22343299,23749970,24752279,26731423