argG [2019-05-21 10:43:51]

argininosuccinate synthase, reversible

Locus: BSU29450

Isoelectric point: 5.03

Molecular weight: 44.66 kDa

Protein length: 403 aa

Gene length: 1212 bp

Function: biosynthesis of arginine

Product: argininosuccinate synthase, reversible

Essential: no

E.C.: 6.3.4.5

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of arginine
• Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

Gene

Coordinates: 3,013,133 3,014,344

The protein

Catalyzed reaction/ biological activity

• ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate (according to Swiss-Prot)

Protein family

• Type 1 subfamily (according to Swiss-Prot)

Modification

• S-cysteinylation after diamide stress (C187) PubMed
• phosphorylated on Arg-262 PubMed

Structure

• 1KH1 (from Thermus thermophilus, 44% identity, 65% similarity) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: argG-argH
  Description: PubMed

  Regulatory mechanism

  • AhrC: repression, in AhrC regulon
  • YlxR: regulation, PubMed, in YlxR regulon

  Regulation

  • repressed by arginine (AhrC)
  • induced in the absence of YlxR PubMed

Biological materials

Mutant

• GP1688 Δ(argH)::aphA3, available in Jörg Stülke's lab
• BKE29450 (argG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATAAAAATCCCCTCTC, downstream forward: _UP4_GCATGAAGAAGCTTTGGGGA
• BKK29450 (argG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATAAAAATCCCCTCTC, downstream forward: _UP4_GCATGAAGAAGCTTTGGGGA

References

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