argG [Mon May 16 2016 16:10:28 GMT+0200 (CEST)]

argininosuccinate synthase, reversible

locus: BSU29450

pI: 5.00

mw: 44.00 kDa

function: biosynthesis of arginine

product: argininosuccinate synthase, reversible

essential: no

ec: 6.3.4.5

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.4|Biosynthesis/ acquisition of arginine]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

[SW|Categories] containing this gene/protein

• [SW|biosynthesis/ acquisition of amino acids], [SW|phosphoproteins], [SW|most abundant proteins]

This gene is a member of the following [SW|regulons]

• [SW|AhrC regulon]

Gene

Coordinates on the chromosome (coding sequence): 3,013,133 -> 3,014,344

The protein

Catalyzed reaction/ biological activity

• ATP L-citrulline L-aspartate = AMP diphosphate N(omega)-(L-arginino)succinate (according to Swiss-Prot)

Protein family

• Type 1 subfamily (according to Swiss-Prot)

Modification

• S-cysteinylation after diamide stress (C187) [Pubmed|17611193]
• phosphorylated on Arg-262 [Pubmed|22517742]

Structure

• [PDB|1KH1] (from ''Thermus thermophilus'', 44% identity, 65% similarity) [Pubmed|11844799]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operon

• ''[protein|search|argG]-[protein|search|argH]'' [Pubmed|22383849]

Regulation

• repressed by casamino acids [Pubmed|12107147]
• repressed by arginine ([protein|search|AhrC])

Regulatory mechanism

• [protein|search|AhrC]: transcription repression

Additional information

• belongs to the 100 [[most abundant proteins]] [PubMed|15378759]

References

• 17611193,22517742,12107147,22383849,15378759