SubtiBank SubtiBank
argG
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argG

argininosuccinate synthase, reversible
Locus
BSU_29450
Isoelectric point
5.03
Molecular weight
44.66 kDa
Protein length
403 aa Sequence Blast
Gene length
1212 bp Sequence Blast
Function
biosynthesis of arginine
Product
argininosuccinate synthase, reversible
Essential
no
E.C.
6.3.4.5
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,013,133 3,014,344

The protein

Catalyzed reaction/ biological activity

  • ATP + L-aspartate + L-citrulline --> 2-(Nω-L-arginino)succinate + AMP + diphosphate + H+ (according to UniProt)
  • Protein family

  • argininosuccinate synthase family (single member, according to UniProt)
  • Modification

  • S-cysteinylation after diamide stress (C187) PubMed
  • phosphorylated on Arg-262 PubMed
  • Structure

  • 1KH1 (from Thermus thermophilus, 44% identity, 65% similarity) PubMed
  • Localization

  • cytoplasm (according to Swiss-Prot)
  • Additional information

  • belongs to the 100 most abundant proteins PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Regulatory mechanism

  • AhrC: repression, in AhrC regulon
  • YlxR: regulation, PubMed, in YlxR regulon
  • Regulation

  • repressed by arginine (AhrC)
  • induced in the absence of YlxR PubMed
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    Biological materials

    Mutant

  • GP1688 Δ(argG-argH)::aphA3, available in Jörg Stülke's lab
  • BKE29450 (argG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATAAAAATCCCCTCTC, downstream forward: _UP4_GCATGAAGAAGCTTTGGGGA
  • BKK29450 (argG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATAAAAATCCCCTCTC, downstream forward: _UP4_GCATGAAGAAGCTTTGGGGA
  • References

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