ogt [2019-07-31 13:02:40]

O6-methylguanine DNA alkyltransferase

locus: BSU_13540

pI: 7.09

mw: 18.61 kDa

protein length: 165 aa

gene length: 498 bp

function: DNA repair

product: O6-methylguanine DNA alkyltransferase

essential: no

ec: 2.1.1.63

synonyms: dat

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]

Gene

Coordinates: 1,421,426 1,421,923

The protein

Catalyzed reaction/ biological activity

• DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine (according to Swiss-Prot)

Protein family

• MGMT family (with [protein|D7E4E4A95CFDB3CFCC7432B08653644AF6876CA1|AdaB], according to UniProt)

Structure

• [PDB|1SFE] (from ''E. coli'', 36% identity, 51% similarity) [Pubmed|8156986]

Expression and Regulation

Operons

• genes: [gene|1582E81F7DA85F38D6732D341ABD0F052587F25A|kinE]-[gene|9492D341F54BE019B21C0BAF7465AD8F84CC6F23|ogt]
  description: [Pubmed|2506524]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2506524], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• BKE13540 ([gene|9492D341F54BE019B21C0BAF7465AD8F84CC6F23|ogt]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGTTTCGGCTGTCGTATAGT, downstream forward: _UP4_TAAAACATATGGCACGTTCC
• BKK13540 ([gene|9492D341F54BE019B21C0BAF7465AD8F84CC6F23|ogt]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGTTTCGGCTGTCGTATAGT, downstream forward: _UP4_TAAAACATATGGCACGTTCC

References

• 2506524,2105461,9366274