SubtiBank SubtiBank
ogt
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ogt

O6-methylguanine DNA alkyltransferase
Locus
BSU_13540
Isoelectric point
7.09
Molecular weight
18.61 kDa
Protein length
165 aa Sequence Blast
Gene length
498 bp Sequence Blast
Function
DNA repair
Product
O6-methylguanine DNA alkyltransferase
Essential
no
E.C.
2.1.1.63
Synonyms
dat
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Genetics → DNA repair/ recombination
  • Information processing → Genetics → DNA repair/ recombination → Other proteins

    • Gene

    Coordinates
    1,421,426 1,421,923

    The protein

    Catalyzed reaction/ biological activity

  • 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] --> 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein] (according to UniProt)
  • 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] --> thymidine in DNA + S-methyl-L-cysteinyl-[protein] (according to UniProt)

    • Protein family

  • MGMT family (with AdaB, according to UniProt)

    • Structure

  • 1SFE (from E. coli, 36% identity, 51% similarity) PubMed

    • Expression and Regulation

    Operons

    Genes
    kinE-ogt
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
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    Biological materials

    Mutant

  • BKE13540 (ogt::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CGTTTCGGCTGTCGTATAGT, downstream forward: _UP4_TAAAACATATGGCACGTTCC
  • BKK13540 (ogt::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CGTTTCGGCTGTCGTATAGT, downstream forward: _UP4_TAAAACATATGGCACGTTCC

    • References

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