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asnH [2019-08-26 13:10:37]
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asnH [2019-08-26 13:10:37]

asparagine synthase (glutamine-hydrolysing)
locus
BSU_39920
pI
5.53
mw
85.66 kDa
protein length
747 aa Sequence Blast
gene length
2244 bp Sequence Blast
function
biosynthesis of asparagine
product
asparagine synthase (glutamine-hydrolysing)
essential
no
ec
6.3.5.4
synonyms
yxaN
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.5|Biosynthesis/ acquisition of aspartate/ asparagine]

    • Gene

    Coordinates
    4,098,926 4,101,169

    The protein

    Catalyzed reaction/ biological activity

  • ATP + H2O + L-aspartate + L-glutamine --> AMP + diphosphate + H+ + L-asparagine + L-glutamate (according to UniProt)

    • Protein family

  • asparagine synthetase family (with [protein|6F5BBCBA02EA1C016AA37EB63D252A4C07CE6617|AsnB] and [protein|15D150B53A25325DBC414F0C2B0D44AEF4B115A9|AsnO], according to UniProt)

    • Structure

  • [PDB|1CT9] (from E. coli, corresponds to aa 30 ... 537, 27% identity) [pubmed|10587437]

    • Expression and Regulation

    Operons

    genes
    [gene|787BB72119DB1321A8281D7C46FED28CAEDBC97C|yxbB]-[gene|88B01CC87394AEE693A98574F9343EBF99F0D60E|yxbA]-[gene|A15368CD02487EBC1AF999FED34672E38167BCB2|yxnB]-[gene|9446D9C88CBBBC5A08495D8AA49A92A9F47F2C9B|asnH]-[gene|BF0E188249161B3CC24D223FDD48B820EBED8F55|yxaM]
    description
    [Pubmed|10746760]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|20185509], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|12618455], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|15101989], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

    • regulation

  • repressed by glucose (8-fold) [Pubmed|12850135]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B689 (asnH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1688 NBRP B. subtilis, Japan]
  • BKE39920 ([gene|9446D9C88CBBBC5A08495D8AA49A92A9F47F2C9B|asnH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGTCTCCCTCCCAATA, downstream forward: _UP4_AATCTGCCAGAAGGAGCATA
  • BKK39920 ([gene|9446D9C88CBBBC5A08495D8AA49A92A9F47F2C9B|asnH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGTCTCCCTCCCAATA, downstream forward: _UP4_AATCTGCCAGAAGGAGCATA

    • References

    Reviews

  • 12859215,11395405

    • Original publications

  • 20185509,10746760,12618455,10498721,12618455,15101989,10587437