asnH

asparagine synthase (glutamine-hydrolysing)

Locus: BSU_39920

Isoelectric point: 5.53

Molecular weight: 85.66 kDa

Protein length: 747 aa

Gene length: 2244 bp

Function: biosynthesis of asparagine

Product: asparagine synthase (glutamine-hydrolysing)

Essential: no

E.C.: 6.3.5.4

Synonyms: yxaN

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of aspartate/ asparagine

Gene

Coordinates: 4,098,926 4,101,169

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + L-aspartate + L-glutamine --> AMP + diphosphate + H⁺ + L-asparagine + L-glutamate (according to UniProt)

Protein family

• asparagine synthetase family (with AsnB and AsnO, according to UniProt)

Domains

• Glutamine amidotransferase type-2 domain (aa 2-218) (according to UniProt)

Structure

• 1CT9 (from E. coli, corresponds to aa 30 ... 537, 27% identity) PubMed

Expression and Regulation

Operons

• Genes: yxbB-yxbA-yxnB-asnH-yxaM
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CodY: repression, PubMed, in CodY regulon
  • AbrB: repression, PubMed, in AbrB regulon

  Regulation

  • repressed by glucose (8-fold) PubMed

Biological materials

Mutant

• MGNA-B689 (asnH::erm), available at the NBRP B. subtilis, Japan
• BKE39920 (asnH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGTCTCCCTCCCAATA, downstream forward: _UP4_AATCTGCCAGAAGGAGCATA
• BKK39920 (asnH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGTCTCCCTCCCAATA, downstream forward: _UP4_AATCTGCCAGAAGGAGCATA

References

Reviews

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Original publications

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