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csrA [2017-12-06 10:43:18]
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csrA [2017-12-06 10:43:18]

motility regulator, binds to the hag mRNA to inhibit its translation
locus
BSU35370
pI
6.09
mw
8.00 kDa
protein length
74 aa Sequence Blast
gene length
222 bp Sequence Blast
function
control of hag translation
product
motility regulator
essential
no
synonyms
yviG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.4|RNA binding regulators]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.1|Signal transduction in motility and chemotaxis] → [category|SW 4.1.1.1.5|Additional chemotaxis signal transduction and regulatory proteins]

    • Gene

    Coordinates
    3,636,046 → 3,636,270

    The protein

    Catalyzed reaction/ biological activity

  • [protein|93F328524989597C7D2329B25E665496C9631E87|CsrA] binds the ''[gene|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag]'' mRNA to inhibit translation [Pubmed|21895793]

    • Protein family

  • csrA family (according to Swiss-Prot)

    • Effectors of protein activity

  • [protein|93F328524989597C7D2329B25E665496C9631E87|CsrA] is sequestered by interaction with [protein|6A6CBD75DCC33C804F853F47D37773930D398CFE|FliW] (the two proteins form a conserved module in many bacteria) [Pubmed|21895793,27516547]

    • Structure

  • [PDB|1T3O]
  • [PDB|5DMB] ([protein|6A6CBD75DCC33C804F853F47D37773930D398CFE|FliW]-[protein|93F328524989597C7D2329B25E665496C9631E87|CsrA] complex) [Pubmed|27551070]

    • Additional information

  • [protein|93F328524989597C7D2329B25E665496C9631E87|CsrA] and [protein|6A6CBD75DCC33C804F853F47D37773930D398CFE|FliW] form a conserved module in many bacteria [Pubmed|21895793]

    • Expression and Regulation

    Operons

    genes
    [gene|45F27C0789199626BA90D2C69E8F13525C911478|comFA]-[gene|D290247A43280532947B707F3AF388D7A7F404D3|comFB]-[gene|04D9D70C0BCFDCBD00B5156908C348135080A9C2|comFC]-[gene|049B1EBF64F0EBC04CE6D529C8EDB0B6B2184DF5|yvyF]-[gene|F03144BF8A187C8931938A21433431B8961E8EE7|flgM]-[gene|6F4CD36D4C1EE99EF7A503F44F53AEB8EFFEAB7F|yvyG]-[gene|767ADBEF1B40CB3C74116CEA8CFB2FAF19C1FE7B|flgK]-[gene|82AB04023BCB57967C7501E6AEAC4BB593AA6480|flgL]-[gene|E5A59D2AF53FA220BA98D9653589A8F0D24F75B8|yviE]-[gene|6A6CBD75DCC33C804F853F47D37773930D398CFE|fliW]-[gene|93F328524989597C7D2329B25E665496C9631E87|csrA]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8412657], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|8045879], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]

    • regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|8412657], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]
  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P, [Pubmed|21736639], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon]
  • [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]: repression, [Pubmed|19898538], in [regulon|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC regulon]
    • view in new tab

    genes
    [gene|93F328524989597C7D2329B25E665496C9631E87|csrA]
    description
    [Pubmed|8969505]

    sigma factors

  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|26577401], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A392 (csrA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/392 NBRP B. subtilis, Japan]
  • GP469 (spc), available in [SW|Stülke] lab
  • BKE35370 (Δ[gene|93F328524989597C7D2329B25E665496C9631E87|csrA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCGTTTATTTTCCGCGATA, downstream forward: _UP4_TGAGGATTTTTTTATTTTTG
  • BKK35370 (Δ[gene|93F328524989597C7D2329B25E665496C9631E87|csrA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCGTTTATTTTCCGCGATA, downstream forward: _UP4_TGAGGATTTTTTTATTTTTG

    • Expression vector

  • pGP381 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Stülke] lab
  • for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP383, available in [SW|Stülke] lab

    • lacZ fusion

  • pGP461 (in [protein|search|pAC7]), available in [SW|Stülke] lab

    • References

    Reviews

  • 19385727,22672726,25251856

    • Original Publications

  • 16822857,17555441,8969505,21895793,23144244,26577401,27516547,27551070