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folE2 [2019-05-29 17:07:31]
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folE2 [2019-05-29 17:07:31]

GTP cyclohydrolase IB, replaces FolE under conditions of zinc starvation
Locus
BSU03340
Isoelectric point
7.83
Molecular weight
34.63 kDa
Protein length
305 aa Sequence Blast
Gene length
915 bp Sequence Blast
Function
biosynthesis of folate
Product
zinc-independent GTP cyclohydrolase IB
Essential
no
E.C.
3.5.4.16
Synonyms
yciA

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
364,259 → 365,173

The protein

Catalyzed reaction/ biological activity

  • GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate (according to Swiss-Prot)
  • Protein family

  • GTP cyclohydrolase IV family (according to Swiss-Prot)
  • Cofactors

  • activated by a variety of divalent cations PubMed
  • Structure

    Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • Zur: repression, in the presence of zinc PubMed, in Zur regulon
  • Regulation

  • folE2: induced by zinc starvation (Zur) PubMed
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    Biological materials

    Mutant

  • BKE03340 (ΔfolEB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCAGAAAACTCCTCTCA, downstream forward: _UP4_TTTTCGAAGGAAGTGGATAA
  • BKK03340 (ΔfolEB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCAGAAAACTCCTCTCA, downstream forward: _UP4_TTTTCGAAGGAAGTGGATAA
  • References

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