folEB

GTP cyclohydrolase IB, required for the initial step of queuosine synthesis (tRNA modification), replaces FolE under conditions of zinc starvation

Locus: BSU_03340

Isoelectric point: 7.83

Molecular weight: 34.63 kDa

Protein length: 305 aa

Gene length: 915 bp

Function: biosynthesis of folate and queuosine

Product: zinc-independent GTP cyclohydrolase IB

Essential: no

E.C.: 3.5.4.16

Synonyms: folE2,yciA

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis of folate
• Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation

Gene

Coordinates: 364,259 → 365,173

The protein

Catalyzed reaction/ biological activity

• GTP + H₂O --> 7,8-dihydroneopterin 3'-triphosphate + formate + H⁺ (according to UniProt)

Protein family

• GTP cyclohydrolase IV family (single member, according to UniProt)

Cofactors

• activated by a variety of divalent cations PubMed

Structure

• 3D1T PubMed

Expression and Regulation

Operons

• Genes: folEB-yciB-yczL-zagA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • Zur: repression, in the presence of zinc PubMed, in Zur regulon

  Regulation

  • folE2: induced by zinc starvation (Zur) PubMed

Biological materials

Mutant

• BKE03340 (ΔfolEB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCAGAAAACTCCTCTCA, downstream forward: _UP4_TTTTCGAAGGAAGTGGATAA
• BKK03340 (ΔfolEB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCAGAAAACTCCTCTCA, downstream forward: _UP4_TTTTCGAAGGAAGTGGATAA

References

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