proI [2017-07-30 17:22:46]

pyrroline-5-carboxylate reductase

locus: BSU23800

pI: 6.70

mw: 30.24 kDa

protein length: 278 aa

gene length: 834 bp

function: biosynthesis of proline

product: pyrroline-5-carboxylate reductase

essential: no

ec: 1.5.1.2

synonyms: yqjO

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.2|Biosynthesis/ acquisition of proline]

Gene

Coordinates: 2,473,151 → 2,473,987

The protein

Catalyzed reaction/ biological activity

• L-proline + NAD(P)⁺ = 1-pyrroline-5-carboxylate + NAD(P)H (according to Swiss-Prot)

Protein family

• pyrroline-5-carboxylate reductase family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|542871C77BE841AA59A65FFE8A44AB85BF9180F0|ProH]

Expression and Regulation

Operons

• genes: [gene|93000B579F630E9F0C6EED40D6661E5E15141FDE|proI]
  description: [Pubmed|21784929]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|21233158], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|T-box|T-box]: anti-termination, in [regulon|T-box|T-box]

  regulation

  • induced by proline limitation ([protein|search|T-box]) [Pubmed|21233158]

Biological materials

Mutant

• MGNA-C395 (yqjO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2393 NBRP B. subtilis, Japan]

References

Reviews

• 25367752

Original publications

• 19258532,21233158,11418582,21784929