cdaS [2017-8-11 16:0:3]

sporulation-specific diadenylate cyclase, synthesis of c-di-AMP

Locus: BSU19430

Isoelectric point: 5.37

Molecular weight: 22.75 kDa

Protein length: 207 aa

Gene length: 621 bp

Function: synthesis of c-di-AMP

Product: sporulation-specific diadenylate cyclase

Essential: no

E.C.: 2.7.7.85

Synonyms: yojJ

Genomic Context

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Categories containing this gene/protein

• Metabolism → Nucleotide metabolism → Metabolism of signalling nucleotides
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

Gene

Coordinates: 2,118,504 → 2,119,127

Phenotypes of a mutant

• a cdaA cdaS disA triple mutant is not viable on complex medium; however, the mutant grows at low potassium concentration (0.1 mM) PubMed

The protein

Catalyzed reaction/ biological activity

• synthesis of c-di-AMP from two molecules of ATP PubMed

Paralogous protein(s)

• CdaA

Domains

• N-terminal autoinhibitory domain PubMed
• C-terminal DAC domain involved in the synthesis of c-di-AMP PubMed

Structure

• 2FB5 (the protein of B. cereus, 48% identity, 81% similarity)

Expression and Regulation

Operons

• Genes: cdaS
  Description: PubMed

  Sigma factors

  • SigG: sigma factor, PubMed, in SigG regulon

  Regulation

  • expressed late during sporulation in the forespore (SigG) PubMed

Biological materials

Mutant

• MGNA-B426 (yojJ::erm), available at the NBRP B. subtilis, Japan
• GP983 (cdaS::ermC), available in Jörg Stülke's lab PubMed
• GP1360 (cdaS::spc), available in Jörg Stülke's lab
• GP2222 (cdaA::cat cdaS::ermC disA::tet), available in Jörg Stülke's lab, the mutant is only viable on minimal medium at low potassium concentration PubMed

Expression vector

• IPTG inducible expression of '''B. cereus' Strep-cdaS in E. coli'': pGP2593 (in pGP172), available in Jörg Stülke's lab

References

Reviews

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Original publications

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