SubtiBank SubtiBank
sleB
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sleB

spore cortex-lytic enzyme
Locus
BSU_22930
Isoelectric point
9.51
Molecular weight
33.85 kDa
Protein length
305 aa Sequence Blast
Gene length
918 bp Sequence Blast
Function
degration of the spore cortex, germination
Product
spore cortex-lytic enzyme
Essential
no
Synonyms
ypeA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Lifestyles → Sporulation → Germination → Additional germination proteins
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    2,399,152 2,400,069

    The protein

    Protein family

  • sleB family (single member, according to UniProt)

    • Domains

  • contains a signal peptide PubMed

    • Structure

  • 4FET (from B. anthracis, 50% identity) PubMed

    • Localization

  • outer surface of the inner spore membrane PubMed

    • Expression and Regulation

    Operons

    Genes
    sleB-ypeB
    Description
    PubMed

    Sigma factors

  • SigG: sigma factor, PubMed, in SigG regulon

    • Regulation

  • expressed late during sporulation in the forespore (SigG) PubMed
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    Biological materials

    Mutant

  • MGNA-A399 (sleB::erm), available at the NBRP B. subtilis, Japan
  • 1G21 ( sleB::spec), PubMed, available at BGSC
  • BKE22930 (sleB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTTCAAGCCTCCTAC, downstream forward: _UP4_TAGCAGATATGAGAAAGCAT
  • BKK22930 (sleB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTTCAAGCCTCCTAC, downstream forward: _UP4_TAGCAGATATGAGAAAGCAT

    • References

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