SubtiBank SubtiBank
ktrA [2017-07-07 13:05:55]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

ktrA [2017-07-07 13:05:55]

high affinity potassium transporter [protein|92D0275E0768780F782F4B3724BC5181E436B6B1|KtrA]-[protein|A287B9771A56D03ADCA603C1969CBF03DCB10E95|KtrB], peripheric membrane component
locus
BSU31090
pI
5.98
mw
24.73 kDa
protein length
222 aa Sequence Blast
gene length
666 bp Sequence Blast
function
potassium uptake
product
high affinity potassium transporter [protein|92D0275E0768780F782F4B3724BC5181E436B6B1|KtrA]-[protein|A287B9771A56D03ADCA603C1969CBF03DCB10E95|KtrB], peripheric membrane component
essential
no
synonyms
yuaA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.3|Potassium uptake/ export]
  • [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.1|Targets of c-di-AMP]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,188,414 → 3,189,082

    Phenotypes of a mutant

  • a [gene|search|kimA ][gene|search|ktrA ][gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB] mutant requires high potassium concentrations on minimal medium with ammonium as nitrogen source [pubmed|28420751,28679749]
  • a [gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB] mutant of ''B. subtilis'' NCIB3610 is reduced in sliding (dendritic spreading) [Pubmed|16321950]

    • The protein

    Paralogous protein(s)

  • [protein|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|KtrC]

    • [SW|Domains]

  • contains a [SW|RCK_N domain] at the N-terminus (according to [http://www.uniprot.org/uniprot/?query=RCK+N-terminal+domain&sort=score UniProt])
  • contains a [SW|RCK_C domain] at the C-terminus (according to [http://www.uniprot.org/uniprot/?query=RCK+C-terminal+domain&sort=score UniProt])

    • Structure

  • [PDB|4J7C] (the [protein|92D0275E0768780F782F4B3724BC5181E436B6B1|KtrA]-[protein|A287B9771A56D03ADCA603C1969CBF03DCB10E95|KtrB] complex) [Pubmed|23598340]
  • [PDB|4XTT] (the ''S. aureus'' KtrA [SW|RCK_C domain] in complex with c-di-AMP, 48% identity) [Pubmed|25957408]
  • [PDB|1LSU] (complex with NADH)
  • [http://www.proteopedia.org/wiki/index.php/2hmw 2HMW] ( complex with ATP)

    • [SW|Localization]

  • peripheral membrane protein [Pubmed|12562800]

    • Expression and Regulation

    Operons

    genes
    [gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]
    description
    [Pubmed|12562800]

    regulatory mechanism

  • [regulon|ydaO riboswitch|ydaO riboswitch]: termination/antitermination, expression is switched off upon binding of c-di-AMP, in [regulon|ydaO riboswitch|ydaO riboswitch]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B543 (yuaA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1542 NBRP B. subtilis, Japan]
  • 1A954 ( ''ktrA''::''kan''), [Pubmed|12562800], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A954&Search=1A954 BGSC]
  • GHB1 (Δ[gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]::''aphA3''), available in [SW|Erhard Bremer]'s lab
  • GP92 (Δ[gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]::''aphA3''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]
  • GP2083 (Δ[gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB]::''aphA3'' Δ[gene|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|ktrC]::''tet''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]

    • Expression vector

  • pGP2594: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • GP2176 (based on [SW|pAC5]), available in [SW|Jörg Stülke]'s lab
  • GP2177 (based on [SW|pAC7]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]

    • References

    Reviews

  • 25869574,27935846,28086088

    • Original publications

  • 12562800,15096624,16321950,20511502,23086297,23598340,24141192,25957408,16990138,26771197,28086091,28420751,28504641,28679749