ktrA [2017-07-07 13:05:55]

high affinity potassium transporter KtrA-KtrB, peripheric membrane component

Locus: BSU31090

Isoelectric point: 5.98

Molecular weight: 24.73 kDa

Protein length: 222 aa

Gene length: 666 bp

Function: potassium uptake

Product: high affinity potassium transporter KtrA-KtrB, peripheric membrane component

Essential: no

Synonyms: yuaA

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Metal ion transporter
• Cellular processes → Homeostasis → Metal ion homeostasis (K, Na, Ca, Mg) → Potassium uptake/ export
• Information processing → Targets of second messengers → Targets of c-di-AMP
• Lifestyles → Coping with stress → Coping with hyper-osmotic stress
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,188,414 → 3,189,082

Phenotypes of a mutant

• a kimA ktrA ktrB mutant requires high potassium concentrations on minimal medium with ammonium as nitrogen source PubMed
• a ktrA-ktrB mutant of B. subtilis NCIB3610 is reduced in sliding (dendritic spreading) PubMed

The protein

Paralogous protein(s)

• KtrC

Domains

• contains a RCK_N domain at the N-terminus (according to UniProt)
• contains a RCK_C domain at the C-terminus (according to UniProt)

Structure

• 4J7C (the KtrA-KtrB complex) PubMed
• 4XTT (the S. aureus KtrA RCK_C domain in complex with c-di-AMP, 48% identity) PubMed
• 1LSU (complex with NADH)
• 2HMW ( complex with ATP)

Localization

• peripheral membrane protein PubMed

Expression and Regulation

Operons

• Genes: ktrA-ktrB
  Description: PubMed

  Regulatory mechanism

  • ydaO riboswitch: termination/antitermination, expression is switched off upon binding of c-di-AMP, in ydaO riboswitch

Biological materials

Mutant

• MGNA-B543 (yuaA::erm), available at the NBRP B. subtilis, Japan
• 1A954 ( ktrA::kan), PubMed, available at BGSC
• GHB1 (ΔktrA-ktrB::aphA3), available in Erhard Bremer's lab
• GP92 (ΔktrA-ktrB::aphA3), available in Jörg Stülke's lab PubMed
• GP2083 (ΔktrA-ktrB::aphA3 ΔktrC::tet), available in Jörg Stülke's lab PubMed

Expression vector

• pGP2594: (IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab

LacZ fusion

• GP2176 (based on pAC5), available in Jörg Stülke's lab
• GP2177 (based on pAC7), available in Jörg Stülke's lab

Labs working on this gene/protein

• Erhard Bremer, University of Marburg, Germany homepage

References

Reviews

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Original publications

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