high affinity potassium transporter KtrA-KtrB, peripheric membrane component (proton symport)
function
potassium uptake
product
high affinity potassium transporter KtrA-KtrB,
peripheric membrane component (proton symport)
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter][category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.3|Potassium uptake/ export][category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.1|Targets of c-di-AMP][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins][SW|Categories] containing this gene/protein
[SW|transporters/ other], [SW|metal ion homeostasis (K, Na, Ca, Mg)], [SW|coping with hyper-osmotic stress], [SW|membrane proteins]This gene is a member of the following [SW|regulons]
[SW|YdaO riboswitch]Gene
Coordinates on the chromosome (coding sequence)
3,188,414 -> 3,189,082
Phenotypes of a mutant
The ''[protein|search|ktrA]-[protein|search|ktrB]'' mutant of ''B. subtilis'' NCIB3610 is reduced in sliding (dendritic spreading) [Pubmed|16321950]The protein
Paralogous protein(s)
[protein|search|KtrC][SW|Domains]
contains a [SW|RCK_N domain] at the N-terminus (according to [http://www.uniprot.org/uniprot/?query=domain:RCK N-terminal domain* UniProt])contains a [SW|RCK_C domain] at the C-terminus (according to [http://www.uniprot.org/uniprot/?query=domain:RCK C-terminal domain* UniProt])Structure
[PDB|4J7C] (the [protein|search|KtrA]-[protein|search|KtrB] complex) [Pubmed|23598340][PDB|4XTT] (the ''S. aureus'' KtrA [SW|RCK_C domain] in complex with c-di-AMP, 48% identity) [Pubmed|25957408][PDB|1LSU] (complex with NADH)[http://www.proteopedia.org/wiki/index.php/2hmw 2HMW] ( complex with ATP)[SW|Localization]
peripheral membrane protein [Pubmed|12562800][SW|Interactions]
[protein|search|KtrA]-[protein|search|KtrB] [Pubmed|12562800], composed of an octameric [protein|search|KtrA] ring and two [protein|search|KtrB] dimers [Pubmed|23598340]Expression and Regulation
Operon
''[protein|search|ktrA]-[protein|search|ktrB]'' [Pubmed|12562800]Regulatory mechanism
expression is controlled via termination antitermination by the [SW|ydaO riboswitch], expression is switched off upon binding of c-di-AMP [Pubmed|24141192,20511502]Biological materials
Mutant
MGNA-B543 (yuaA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1542 NBRP B. subtilis, Japan]1A954 ( ''ktrA''::''kan''), [Pubmed|12562800], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A954&Search=1A954 BGSC]GHB1 (D(''[protein|search|ktrA]-[protein|search|ktrB]'')::''aphA3''), available in [SW|Erhard Bremer]'s labGP92 (D(''[protein|search|ktrA]-[protein|search|ktrB]'')::''aphA3''), available in [SW|Jörg Stülke]'s labGP2083 (D(''[protein|search|ktrA]-[protein|search|ktrB]'')::''aphA3'' D''[protein|search|ktrC]''::''tet''), available in [SW|Jörg Stülke]'s labExpression vector
pGP2594: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lablacZ fusion
GP2176 (based on [SW|pAC5]), available in [SW|Jörg Stülke]'s labGP2177 (based on [SW|pAC7]), available in [SW|Jörg Stülke]'s labLabs working on this gene/protein
[SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]References
Reviews
25869574,27935846,28086088 Original publications
12562800,15096624,16321950,20511502,23086297,23598340,16990138,24141192,25957408,16990138,26771197,28086091 
