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ktrA [2019-04-16 14:27:12]
high affinity potassium channel
KtrA-
KtrB, peripheric membrane component
Molecular weight
24.73 kDa
Product
high affinity potassium channel
KtrA-
KtrB, peripheric membrane component
Genomic Context
Categories containing this gene/protein
Gene
Coordinates
3,188,414 3,189,082
Phenotypes of a mutant
a kimA ktrA ktrB mutant requires high potassium concentrations on minimal medium with ammonium as nitrogen source PubMeda ktrA-ktrB mutant of B. subtilis NCIB3610 is reduced in sliding (dendritic spreading) PubMed The protein
Paralogous protein(s)
Kinetic information
Domains
Effectors of protein activity
Structure
Localization
peripheral membrane protein PubMed Expression and Regulation
Operons
Additional information
growth at extreme potassium limitation results in the acquisition of promoter mutations with increased ktrA-ktrB expression PubMed Biological materials
Mutant
MGNA-B543 (yuaA::erm), available at the NBRP B. subtilis, Japan1A954 ( ktrA::kan), PubMed, available at BGSCGHB1 (ktrA-ktrB::aphA3), available in Erhard Bremer's labGP92 (ktrA-ktrB::aphA3), available in Jörg Stülke's lab PubMedGP2083 (ktrA-ktrB::aphA3 ktrC::tet), available in Jörg Stülke's lab PubMedGP2498 (ktrA-ktrB::spc kimA::cat), available in Jörg Stülke's labBKE31090 (ktrA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATGTTCATATCTCCCTTA, downstream forward: _UP4_GAAAACGAAGGGATGTAGACBKK31090 (ktrA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATGTTCATATCTCCCTTA, downstream forward: _UP4_GAAAACGAAGGGATGTAGACGP2716 (ktrA-ktrB::spc), available in Jörg Stülke's lab Expression vectors
pGP2594: (IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab LacZ fusion
References
Reviews
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Original publications
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Labs working on this gene/protein