ktrA [Fri Dec 16 2016 19:27:59 GMT+0100 (CET)]

high affinity potassium transporter KtrA-KtrB, peripheric membrane component (proton symport)

Locus: BSU31090

Isoelectric point: 5.00

Molecular weight: 24.00 kDa

Function: potassium uptake

Product: high affinity potassium transporter KtrA-KtrB,
peripheric membrane component (proton symport)

Essential: no

Synonyms: yuaA

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Metal ion transporter
• Cellular processes → Homeostasis → Metal ion homeostasis (K, Na, Ca, Mg) → Potassium uptake/ export
• Information processing → Targets of second messengers → Targets of c-di-AMP
• Lifestyles → Coping with stress → Coping with hyper-osmotic stress
• Groups of genes → Membrane proteins

Categories containing this gene/protein

• transporters/ other, metal ion homeostasis (K, Na, Ca, Mg), coping with hyper-osmotic stress, membrane proteins

This gene is a member of the following regulons

• YdaO riboswitch

Gene

Coordinates on the chromosome (coding sequence): 3,188,414 -> 3,189,082

Phenotypes of a mutant

• The ktrA-ktrB mutant of B. subtilis NCIB3610 is reduced in sliding (dendritic spreading) PubMed

The protein

Paralogous protein(s)

• KtrC

Domains

• contains a RCK_N domain at the N-terminus (according to [http://www.uniprot.org/uniprot/?query=domain:RCK N-terminal domain* UniProt])
• contains a RCK_C domain at the C-terminus (according to [http://www.uniprot.org/uniprot/?query=domain:RCK C-terminal domain* UniProt])

Structure

• 4J7C (the KtrA-KtrB complex) PubMed
• 4XTT (the S. aureus KtrA RCK_C domain in complex with c-di-AMP, 48% identity) PubMed
• 1LSU (complex with NADH)
• 2HMW ( complex with ATP)

Localization

• peripheral membrane protein PubMed

Interactions

• KtrA-KtrB PubMed, composed of an octameric KtrA ring and two KtrB dimers PubMed

Expression and Regulation

Operon

• ktrA-ktrB PubMed

Regulatory mechanism

• expression is controlled via termination antitermination by the ydaO riboswitch, expression is switched off upon binding of c-di-AMP PubMed

Biological materials

Mutant

• MGNA-B543 (yuaA::erm), available at the NBRP B. subtilis, Japan
• 1A954 ( ktrA::kan), PubMed, available at BGSC
• GHB1 (D(ktrA-ktrB)::aphA3), available in Erhard Bremer's lab
• GP92 (D(ktrA-ktrB)::aphA3), available in Jörg Stülke's lab
• GP2083 (D(ktrA-ktrB)::aphA3 DktrC::tet), available in Jörg Stülke's lab

Expression vector

• pGP2594: (IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab

LacZ fusion

• GP2176 (based on pAC5), available in Jörg Stülke's lab
• GP2177 (based on pAC7), available in Jörg Stülke's lab

Labs working on this gene/protein

• Erhard Bremer, University of Marburg, Germany homepage

References

Reviews

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Original publications

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