ylnD

probable uroporphyrin-III C-methyltransferase

Locus: BSU_15610

Isoelectric point: 5.75

Molecular weight: 28.37 kDa

Protein length: 257 aa

Gene length: 774 bp

Function: siroheme biosynthesis , sulfite reduction

Product: probable uroporphyrin-III C-methyltransferase

Essential: no

E.C.: 2.1.1.107

Synonyms: sumT

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis of heme/ siroheme

Gene

Coordinates: 1,634,061 1,634,834

The protein

Catalyzed reaction/ biological activity

• 2 S-adenosyl-L-methionine + uroporphyrinogen III --> H⁺ + precorrin-2 + 2 S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• precorrin methyltransferase family (with NasF, according to UniProt)

Paralogous protein(s)

• NasF:

Structure

• 1PJQ (CysG from Salmonella enterica, 46% identity) PubMed

Expression and Regulation

Operons

• Genes: cysH-cysP-sat-cysC-ylnD-sirB-ylnF
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CymR: repression, in CymR regulon
  • S-box: termination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box

  Regulation

  • induced by methionine starvation (S-box) PubMed
  • the S-box RNA is degraded by RNase Y PubMed

Biological materials

Mutant

• MGNA-B368 (ylnD::erm), available at the NBRP B. subtilis, Japan
• BKE15610 (ylnD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGTATTTCCCCCGATCT, downstream forward: _UP4_GATTTAAGCGAGGCGTTGTA
• BKK15610 (ylnD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGTATTTCCCCCGATCT, downstream forward: _UP4_GATTTAAGCGAGGCGTTGTA

References

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