slrR [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

transcriptional activator (Xre family) of competence development and sporulation genes, represses SigD-dependent flagellar genes, antagonist of SlrA and SinR, has LexA-like autocleavage activity

Locus: BSU34380

Isoelectric point: 9.00

Molecular weight: 17.00 kDa

Function: regulation of initiation of biofilm formation and of autolysis

Product: transcriptional regulator (Xre family), SlrA antagonist

Essential: no

Synonyms: yveJ, slr

Genomic Context

Loading

Categories containing this gene/protein

• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other
• Information processing → Regulation of gene expression → Transition state regulators
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Regulation
• Lifestyles → Exponential and early post-exponential lifestyles → Swarming

Categories containing this gene/protein

• transcription factors and their control, transition state regulators, biofilm formation

This gene is a member of the following regulons

• Abh regulon, AbrB regulon, SinR regulon

Gene

Coordinates on the chromosome (coding sequence): 3,530,101 -> 3,530,559

Phenotypes of a mutant

• smooth colonies on MsGG medium, no biofilm formation PubMed

The protein

Catalyzed reaction/ biological activity

• SlrR binds to and inhibits the activity of SlrA, SlrA indirectly stimulates the synthesis of SlrR by interacting with SinR. SlrR can bind to SinR and SinR directly represses the transcription of SlrR. SlrR indirectly derepresses its own gene. The heterocomplex of SlrR-SinR is a repressor of autolysin and motility genes and inhibits the repressor function of SinR. PubMed
• repression of transcription of lytA-lytB-lytC and lytF PubMed
• autocleavage PubMed

Protein family

• Xre family

Paralogous protein(s)

• SinR

Modification

• subject to self-cleavage via a LexA-like autopeptidase, this involves ClpC and requires DegU PubMed

Effectors of protein activity

• interaction with SinR triggers binding of SlrR to the promoters of lytA-lytB-lytC and lytF, resulting in their repression PubMed

Interactions

• SlrA-SlrR PubMed
• SlrR-SinR PubMed, K(D) 47.5 nM PubMed, Veg may inhibit this interaction PubMed

Expression and Regulation

Operon

• slrR PubMed

Regulatory mechanism

• Abh: transcription activation PubMed
• SinR: transcription repression PubMed
• AbrB: transcription repression PubMed

Additional information

• induction by sequestration of SinR by SinI, SlrA PubMed or by SlrR itself PubMed
• the 'slrR' gene is not expressed in a 'ymdB' mutant PubMed
• the amount of the mRNA is substantially decreased upon depletion of [Rny|search|RNase Y] (this is likely due to the increased stability of the 'sinR' mRNA) PubMed

Biological materials

Mutant

• GP955 (slrR-pnbA::cat), available in Jörg Stülke's lab

Expression vector

• pGP2905: expression in E. coli, with N-terminal His-tag, in pWH844, available in Jörg Stülke' lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke' lab

References

Reviews

Loading

Original publications

Loading