iolU

minor NADP-dependent scyllo-inositol dehydrogenase

Locus: BSU_31170

Isoelectric point: 5.15

Molecular weight: 36.36 kDa

Protein length: 328 aa

Gene length: 987 bp

Function: utilization of scyllo-inosose

Product: minor NADP-dependent scyllo-inositol dehydrogenase

Essential: no

Synonyms: yulF

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of inositol

Gene

Coordinates: 3,196,906 3,197,892

The protein

Catalyzed reaction/ biological activity

• NADPH-dependent conversion of scyllo-inosose to scyllo-inositol PubMed
• NADP⁺ + scyllo-inositol --> H⁺ + NADPH + scyllo-inosose (according to UniProt)

Protein family

• Gfo/Idh/MocA family (according to UniProt)

Paralogous protein(s)

• IolG, YrbE, IolX, YfiI, NtdC, YteT, IolW

Cofactors

• NADP⁺ PubMed

Structure

• 4NHE (oxidoreductase from Streptococcus pneumoniae)

Expression and Regulation

Operons

• Genes: iolU
  Description: PubMed

Biological materials

Mutant

• MGNA-A228 (yulF::erm), available at the NBRP B. subtilis, Japan
• BKE31170 (iolU::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTCGTATCGCTCCTTTTG, downstream forward: _UP4_TAAAAAAATCCGCCCGCGTG
• BKK31170 (iolU::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTCGTATCGCTCCTTTTG, downstream forward: _UP4_TAAAAAAATCCGCCCGCGTG

References

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