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ywlF [Wed Jan 20 2016 13:20:09 GMT+0100 (CET)]
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ywlF [Wed Jan 20 2016 13:20:09 GMT+0100 (CET)]

ribose-5-phosphate isomerase
locus
BSU36920
pI
5.00
mw
16.00 kDa
function
pentose phosphate pathway
product
ribose-5-phosphate isomerase
essential
no
synonyms
ipc-32d

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.3|Pentose phosphate pathway]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes]
  • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism]
  • This gene is a member of the following [SW|regulons]

  • [SW|TnrA regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    3,791,199 -> 3,791,648

    The protein

    Protein family

  • lacAB/rpiB family (according to Swiss-Prot)
  • Kinetic information

  • Reversible Mass Action Kinetics [Pubmed|956158] [Pubmed|7144591]
  • Effectors of protein activity

  • Inhibited by 6-phosphogluconate and AMP [Pubmed|7144591]
  • Inhibited by divalent ions such as Ag2, Mg2, Co2 and Zn2 [Pubmed|956158]
  • Activated by EDTA and 2-mercaptoethanol [Pubmed|7144591]
  • Structure

  • [PDB|3HEE] (from ''Clostridium thermocellum'', 55% identity, 72% similarity)
  • Additional information

  • The enzyme has probably more than one active site, but with no cooperativity described [Pubmed|7144591]
  • Expression and Regulation

    Operon

  • ''[protein|search|ywlF]-[protein|search|ywlG]'' [Pubmed|12823818]
  • Regulation

  • repressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|12823818]
  • Regulatory mechanism

  • [protein|search|TnrA]: transcription repression [Pubmed|12823818]
  • Biological materials

    FLAG-tag construct

  • GP1404 (spc, based on [SW|pGP1331]), available in the [SW|Stülke] lab
  • Antibody

  • **
  • References

  • 25755103,12823818,19446032,956158,7144591,956158