ywlF [Wed Jan 20 2016 13:20:09 GMT+0100 (CET)]

ribose-5-phosphate isomerase

Locus: BSU36920

Isoelectric point: 5.00

Molecular weight: 16.00 kDa

Function: pentose phosphate pathway

Product: ribose-5-phosphate isomerase

Essential: no

Synonyms: ipc-32d

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Carbon core metabolism → Pentose phosphate pathway
• Information processing → Genetics → Newly identified competence genes

Categories containing this gene/protein

• carbon core metabolism

This gene is a member of the following regulons

• TnrA regulon

Gene

Coordinates on the chromosome (coding sequence): 3,791,199 -> 3,791,648

The protein

Protein family

• lacAB/rpiB family (according to Swiss-Prot)

Kinetic information

• Reversible Mass Action Kinetics PubMed PubMed

Effectors of protein activity

• Inhibited by 6-phosphogluconate and AMP PubMed
• Inhibited by divalent ions such as Ag2, Mg2, Co2 and Zn2 PubMed
• Activated by EDTA and 2-mercaptoethanol PubMed

Structure

• 3HEE (from Clostridium thermocellum, 55% identity, 72% similarity)

Additional information

• The enzyme has probably more than one active site, but with no cooperativity described PubMed

Expression and Regulation

Operon

• ywlF-ywlG PubMed

Regulation

• repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

Regulatory mechanism

• TnrA: transcription repression PubMed

Biological materials

FLAG-tag construct

• GP1404 (spc, based on pGP1331), available in the Stülke lab

Antibody

• **

References

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