You are currently viewing an outdated version of
SubtiWiki.
Please use
the newest version!
ywlF
ribose-5-phosphate isomerase
Molecular weight
16.07 kDa
Function
pentose phosphate pathway
Product
ribose-5-phosphate isomerase
Genomic Context
Categories containing this gene/protein
Gene
Coordinates
3,791,199 3,791,648
Phenotypes of a mutant
The protein
Protein family
LacAB/RpiB family (single member, according to UniProt)Kinetic information
Effectors of protein activity
Inhibited by 6-phosphogluconate and AMP PubMedInhibited by divalent ions such as Ag2+, Mg2+, Co2+ and Zn2+ PubMedActivated by EDTA and 2-mercaptoethanol PubMed Structure
3HEE (from Clostridium thermocellum, 55% identity, 72% similarity) PubMed Additional information
The enzyme has probably more than one active site, but with no cooperativity described PubMed Expression and Regulation
Operons
Regulatory mechanism
Regulation
repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed view in new tabBiological materials
Mutant
BKE36920 (ywlF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTGCTTTTCCTCCCAGC, downstream forward: _UP4_AAAAACCTGTAGGGGTGCTGBKK36920 (ywlF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTGCTTTTCCTCCCAGC, downstream forward: _UP4_AAAAACCTGTAGGGGTGCTG FLAG-tag construct
Antibody
**References
Loading