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ywlF
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ywlF

ribose-5-phosphate isomerase
Locus
BSU_36920
Isoelectric point
5.42
Molecular weight
16.07 kDa
Protein length
149 aa Sequence Blast
Gene length
450 bp Sequence Blast
Function
pentose phosphate pathway
Product
ribose-5-phosphate isomerase
Essential
no
E.C.
5.3.1.-
Synonyms
ipc-32d

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,791,199 3,791,648

Phenotypes of a mutant

  • poor growth PubMed
  • poorly transformable PubMed
  • The protein

    Protein family

  • LacAB/RpiB family (single member, according to UniProt)
  • Kinetic information

  • Reversible Mass Action Kinetics PubMed PubMed
  • Effectors of protein activity

  • Inhibited by 6-phosphogluconate and AMP PubMed
  • Inhibited by divalent ions such as Ag2+, Mg2+, Co2+ and Zn2+ PubMed
  • Activated by EDTA and 2-mercaptoethanol PubMed
  • Structure

  • 3HEE (from Clostridium thermocellum, 55% identity, 72% similarity) PubMed
  • Additional information

  • The enzyme has probably more than one active site, but with no cooperativity described PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Regulatory mechanism

  • TnrA: repression, PubMed, in TnrA regulon
  • Regulation

  • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
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    Biological materials

    Mutant

  • BKE36920 (ywlF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTGCTTTTCCTCCCAGC, downstream forward: _UP4_AAAAACCTGTAGGGGTGCTG
  • BKK36920 (ywlF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTGCTTTTCCTCCCAGC, downstream forward: _UP4_AAAAACCTGTAGGGGTGCTG
  • FLAG-tag construct

  • GP1404 (spc, based on pGP1331), available in Jörg Stülke's lab
  • Antibody

  • **
  • References

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