SubtiBank SubtiBank
hypR [Tue Dec 13 2016 11:16:37 GMT+0100 (CET)]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

hypR [Tue Dec 13 2016 11:16:37 GMT+0100 (CET)]

MarR/DUF24 family transcription regulator, positively controls the nitroreductase gene hypO in response to disulfide stress
locus
BSU40540
pI
8.42
mw
14.00 kDa
function
control of the nitroreductase gene hypO in response to disulfide stress (diamide, NaOCl)
product
MarR/DUF24 family transcription regulator HypR
essential
no
synonyms
yybR
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

    • [SW|Categories] containing this gene/protein

  • [SW|transcription factors and their control], [SW|resistance against oxidative and electrophile stress]

    • This gene is a member of the following [SW|regulons]

  • [SW|HypR regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    4,167,622 -> 4,167,999

    The protein

    Protein family

  • [[MarR/DUF24 family]]

    • Paralogous protein(s)

  • [protein|search|YdeP],[protein|search|YkvN]

    • Kinetic information

  • Cys14 redox sensing Cys, has lower pKa of 6.36 [Pubmed|22238377]

    • [SW|Domains]

  • 5 alpha helices, 2 beta sheets, MarR-fold with wHTH motif, alpha4 major groove recognition helix, beta2 and 3 form the wing; alpha5 dimer interface [Pubmed|22238377]

    • Modification

  • oxidized to Cys14-Cys49' intersubunit disulfides by disulfide stress [Pubmed|22238377]
  • Cys14 and Cys49' are about 8-9 Angström apart in reduced HypR-Dimer, oxidation moves the major groove recognition alpha4 helices of the HypR dimer about 4 Angstroem towards each other that leads to activation of HypR [Pubmed|22238377]

    • Structure

  • [PDB|4A5N] (reduced HypRC14S dimer) [Pubmed|22238377]
  • [PDB|4A5M] (oxidized HypR C14-C49' intersubunit disulfide-linked dimer ) [Pubmed|22238377]

    • [SW|Localization]

  • cytoplasmic

    • Expression and Regulation

    Operon

  • ''hypR'' [Pubmed|22238377]

    • Regulation

  • activated by disulfide stress conditions (diamide, NaOCl) ''in vivo'' and ''in vitro '' (autoregulation) [Pubmed|22238377]

    • Regulatory mechanism

  • [protein|search|HypR]: transcription activation (autoregulation)[Pubmed|22238377]

    • Labs working on this gene/protein

  • [SW|Haike Antelmann],University of Greifswald, Germany