spoIIID [2019-09-25 11:25:31]

transcriptional regulator (repressor or activator) of a subset of sigma E-dependent genes

Locus: BSU_36420

Isoelectric point: 9.50

Molecular weight: 10.66 kDa

Protein length: 93 aa

Gene length: 282 bp

Function: regulation of mother cell gene expression

Product: transcriptional regulator

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

Gene

Coordinates: 3,748,421 3,748,702

The protein

Catalyzed reaction/ biological activity

• binds its DNA targets as a monomeric protein (possible due to the two DNA-binding motifs) PubMed

Domains

• two DNA-binding regions: HTH at the N-terminus and a basic region near the C-terminus PubMed

Structure

• 2L0K (complex with DNA)

Additional information

• information on binding sites can be found in the PRODORIC2 database

Expression and Regulation

Operons

• Genes: usd-spoIIID-mbl
  Description: PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

  Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed

  Additional information

  • An [ncRNA|search|antisense RNA] is predicted for 'mbl' PubMed

Biological materials

Mutant

• BKE36420 (spoIIID::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ATCGTGCACACCACTCGACC, downstream forward: _UP4_TAATCACCTGGCATTGCCTT
• BKK36420 (spoIIID::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ATCGTGCACACCACTCGACC, downstream forward: _UP4_TAATCACCTGGCATTGCCTT

References

Reviews

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Original Publications

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