codY [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

regulation of a large regulon (more than 100 genes and operons) in response to branched-chain amino acid limitation

Locus: BSU16170

Isoelectric point: 4.00

Molecular weight: 28.00 kDa

Function: regulation of a large regulon in response to branched-chain amino acid limitation

Product: transcriptional pleiotropic repressor

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other
• Information processing → Regulation of gene expression → Regulators of core metabolism
• Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

Categories containing this gene/protein

• transcription factors and their control, regulators of core metabolism, phosphoproteins

This gene is a member of the following regulons

• CodY regulon

Gene

Coordinates on the chromosome (coding sequence): 1,690,119 -> 1,690,898

Phenotypes of a mutant

• no swarming motility on B medium. PubMed
• the mutation suppresses the mucoid phenotype of motA or motB mutants due to loss of DegU phosphorylation and concomitant reduced expression of the capB-capC-capA-capE operon PubMed
• inactivation of codY suppresses the requirement of a relA sasA sasB triple mutant for branched chain amino acids, methionine and threonine PubMed

The protein

Protein family

• codY family (according to Swiss-Prot)

Domains

• contains a GAF domain (ligand binding domain)

Modification

• phosphorylation on Ser-215 PubMed

Effectors of protein activity

• GTP and branched chained amino acids (BCAA) increase the affinity of CodY for its DNA target sequences PubMed

Structure

• 2B0L (C-terminal DNA-binding domain), 2GX5 (N-terminal Gaf domain)

Localization

• cytoplasm (according to Swiss-Prot)

Interactions

• active as dimer (according to PubMed)
• CcpA-CodY PubMed
• RpoA-CodY PubMed

Expression and Regulation

Operon

• codV-clpQ-clpY-codY PubMed

Sigma factor

• SigA PubMed

Regulation

• repressed during growth in the presence of branched chain amino acids (CodY) PubMed

Regulatory mechanism

• CodY: transcription repression PubMed

Additional information

• the intracellular concentration of CodY is about 2.5 myM (according to PubMed)

Biological materials

Mutant

• GP566, available in Jörg Stülke's lab
• a codY::erm mutant is available in Linc Sonenshein's lab
• a codY::spc (BB1043) mutant is available in Linc Sonenshein's, Fabian Commichau's and Jörg Stülke's labs
• BKE16170 (codY::erm trpC2) is available from the Bacillus Genetic Stock Center

Expression vector

• for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP245, available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab

Labs working on this gene/protein

• Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
• Tony Wilkinson, York University, U.K. Homepage
• Oscar Kuipers, University of Groningen, The Netherlands, Homepage

References

Reviews

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The CodY regulon

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Original Publications

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