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sdhB [2019-02-04 16:07:30]
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sdhB [2019-02-04 16:07:30]

succinate dehydrogenase
Locus
BSU28430
Isoelectric point
7.99
Molecular weight
28.27 kDa
Protein length
253 aa Sequence Blast
Gene length
762 bp Sequence Blast
Function
TCA cycle
Product
succinate dehydrogenase (iron-sulfur protein)
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,905,571 2,906,332

The protein

Catalyzed reaction/ biological activity

  • Succinate acceptor = fumarate reduced acceptor (according to Swiss-Prot)
  • Protein family

  • succinate dehydrogenase/fumarate reductase iron-sulfur protein family (according to Swiss-Prot)
  • Cofactors

  • Fe-S cluster PubMed
  • Effectors of protein activity

  • Inhibited by 2-(n-Heptyl)-4-hydroxy-quinoline N-oxide PubMed
  • Activated by Cytochrome b558 PubMed
  • Structure

  • 1NEK (E. coli) PubMed
  • Localization

  • attached to the membrane PubMed
  • Additional information

  • This enzyme is a trimer membrane-bound PubMed PubMed
  • One subunit is bound to citochrome b558, and this subunit is the one bound to the cytosolic side of the membrane PubMed PubMed
  • Another subunit is the flavoprotein one, required for FAD usage PubMed PubMed
  • The other subunit has an iron-sulphur domain necessary for the catalytic activity PubMed PubMed
  • extensive information on the structure and enzymatic properties of succinate dehydrogenase can be found at Proteopedia
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulation

  • constitutive
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    Other regulations

  • FsrA: translation inhibition, PubMed
  • Biological materials

    Mutant

  • GP792 ∆(sdhC-sdhA-sdhB)::phleo, available in Jörg Stülke's lab
  • GP2342 ∆(sdhC-sdhA-sdhB)::kan, Cre-recombinase is integrated in sacA, available in Jörg Stülke's lab
  • GP2343 ∆(sdhC-sdhA-sdhB)::lox72, Cre-recombinase is integrated in sacA, available in Jörg Stülke's lab
  • BKE28430 (sdhB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TATGGTTTTTTGTTCACTCA, downstream forward: _UP4_TAAGAAGAAAAAACCTCTTC
  • BKK28430 (sdhB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TATGGTTTTTTGTTCACTCA, downstream forward: _UP4_TAAGAAGAAAAAACCTCTTC
  • GFP fusion

  • GP1436 (spc, based on pGP1870), available in the Stülke lab
  • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • FLAG-tag construct

  • GP1426 (spc, based on pGP1331), available in the Stülke lab
  • Antibody

  • **
  • References

    Reviews

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    Original publications

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