sdhB [2017-8-11 16:0:3]

succinate dehydrogenase

locus: BSU28430

pI: 7.99

mw: 28.27 kDa

protein length: 253 aa

gene length: 759 bp

function: TCA cycle

product: succinate dehydrogenase (iron-sulfur protein)

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,905,571 → 2,906,332

The protein

Catalyzed reaction/ biological activity

• Succinate acceptor = fumarate reduced acceptor (according to Swiss-Prot)

Protein family

• succinate dehydrogenase/fumarate reductase iron-sulfur protein family (according to Swiss-Prot)

[SW|Cofactors]

• [3 Fe- 4 S] cluster

Effectors of protein activity

• Inhibited by 2-(n-Heptyl)-4-hydroxy-quinoline N-oxide [Pubmed|3910107]
• Activated by Cytochrome b558 [Pubmed|6799760]

Structure

• [PDB|1NEK] (''E. coli'')

[SW|Localization]

• attached to the membrane [Pubmed|18763711]

Additional information

• This enzyme is a trimer membrane-bound [Pubmed|3910107] [Pubmed|6799760]
• One subunit is bound to citochrome b558, and this subunit is the one bound to the cytosolic side of the membrane [Pubmed|3910107] [Pubmed|6799760]
• Another subunit is the flavoprotein one, required for FAD usage [Pubmed|3910107] [Pubmed|6799760]
• The other subunit has an iron-sulphur domain necessary for the catalytic activity [Pubmed|3910107] [Pubmed|6799760]
• extensive information on the structure and enzymatic properties of succinate dehydrogenase can be found at [http://www.proteopedia.org/wiki/index.php/Succinate_Dehydrogenase Proteopedia]

Expression and Regulation

Operons

• genes: [gene|81429F7802CD4B1F7DFDB6C900D7DA5ECA6B9F3F|sdhC]-[gene|0E1FAF4B3A967CC3882B3DDB3CA2D01FD70A0F7E|sdhA]-[gene|901F90AFC94CC7140709845333416106D043269D|sdhB]
  description: [Pubmed|3036777]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2495271], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulation

  • constitutive

Other regulations

• [protein|CE542AE1A32CCD1ABB259E6159F7A37A8078E189|FsrA]: translation inhibition, [Pubmed|22389480]

Biological materials

Mutant

• GP792 (''[gene|81429F7802CD4B1F7DFDB6C900D7DA5ECA6B9F3F|sdhC]-[gene|0E1FAF4B3A967CC3882B3DDB3CA2D01FD70A0F7E|sdhA]-[gene|901F90AFC94CC7140709845333416106D043269D|sdhB]''::''phleo''), available in [SW|Jörg Stülke]'s lab
• GP2342 (''[gene|81429F7802CD4B1F7DFDB6C900D7DA5ECA6B9F3F|sdhC]-[gene|0E1FAF4B3A967CC3882B3DDB3CA2D01FD70A0F7E|sdhA]-[gene|901F90AFC94CC7140709845333416106D043269D|sdhB]''::''kan''), Cre-recombinase is integrated in ''[gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA]'', available in [SW|Jörg Stülke]'s lab
• GP2343 (''[gene|81429F7802CD4B1F7DFDB6C900D7DA5ECA6B9F3F|sdhC]-[gene|0E1FAF4B3A967CC3882B3DDB3CA2D01FD70A0F7E|sdhA]-[gene|901F90AFC94CC7140709845333416106D043269D|sdhB]''::''lox72''), Cre-recombinase is integrated in ''[gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA]'', available in [SW|Jörg Stülke]'s lab

GFP fusion

• GP1436 (spc, based on [SW|pGP1870]), available in the [SW|Stülke] lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Stülke] lab

FLAG-tag construct

• GP1426 (spc, based on [SW|pGP1331]), available in the [SW|Stülke] lab

Antibody

• **

References

Reviews

• 11803018

Original publications

• 1707123,6401289,1324713,3036777,2495271,3027051,7748886,2837411,12560550,3910107,6799760,22389480,23880299