bioI

cytochrome P450 enzyme

locus: BSU_30190

pI: 6.20

mw: 44.70 kDa

protein length: 395 aa

gene length: 1188 bp

function: biosynthesis of biotin

product: cytochrome P450 enzyme

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.1|Biosynthesis/ acquisition of biotin]

Gene

Coordinates: 3,089,226 3,090,413

The protein

Catalyzed reaction/ biological activity

• formation of pimelic acid via a multiple-step oxidative cleavage of long-chain fatty acids
• C₂-C₈-saturated long-chain fatty acyl-[ACP] + 3 O₂ + 2 reduced [flavodoxin] --> fatty aldehyde + 3 H⁺ + 3 H₂O + 2 oxidized [flavodoxin] + pimeloyl-[ACP] (according to UniProt)

Protein family

• [SW|cytochrome P450] family (according to UniProt)

Paralogous protein(s)

• [protein|A95C28DEE73AD25E28C45B88A7C03C92835F9705|CypA], [protein|D8E72C2523D5E6214C9CBAC2C1B27EA45378E162|YjiB], [protein|EF0F13BB682791E167EFC350BF029B08E8A1F410|PksS]

Structure

• [PDB|3EJB], [PDB|3EJE] (complex with [protein|8D260693D0C69442BCB9F3D8480E8425ACFE34D2|AcpA]) [Pubmed|18838690]

Expression and Regulation

Operons

• genes: [gene|C40116089BAD3F9831F8C837DC4F2E045753ABDE|bioW]-[gene|68C86FED3575B864C576DFB73FE7B5BF83994D1F|bioA]-[gene|8B6A3E86A88928526D94FDA94B9C81ABF6E9ACFB|bioF]-[gene|D9A71DAF949FB6C9C22C8361D83C5A0741184977|bioD]-[gene|5880617EB95FC0D19219A868D6D0A3D1D0D9C532|bioB]-[gene|8FBC9A1AC108DC99E275D7A27C18C797D3CDB936|bioI]-[gene|089A32D08B5A7E552CBD9CB9745BD542863B4558|ytbQ]
  description: [Pubmed|8763940,8892842]

  regulatory mechanism

  • [protein|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|BirA]: repression, [Pubmed|8763940,8892842], in [regulon|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|BirA regulon]

  regulation

  • repressed in the presence of biotin ([protein|search|BirA]) [Pubmed|8763940,8892842]

Biological materials

Mutant

• MGNA-A804 (bioI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/804 NBRP B. subtilis, Japan]
• BKE30190 ([gene|8FBC9A1AC108DC99E275D7A27C18C797D3CDB936|bioI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTGATTTTCTCCTTTCT, downstream forward: _UP4_TAAGCCTAAGAATGTGAGTG
• BKK30190 ([gene|8FBC9A1AC108DC99E275D7A27C18C797D3CDB936|bioI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTGATTTTCTCCTTTCT, downstream forward: _UP4_TAAGCCTAAGAATGTGAGTG

References

Reviews

• 21437340

Original publications

• 18838690,8763940,8892842,15449931,15449930,12538057,20658980,11368323,11472016,14737344,12943422,28196402,28898812