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thiE
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thiE

thiamine-phosphate pyrophosphorylase
locus
BSU_38290
pI
5.06
mw
23.53 kDa
protein length
222 aa Sequence Blast
gene length
669 bp Sequence Blast
function
biosynthesis of thiamine by 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate to yield thiamine phosphate
product
thiamine-phosphate pyrophosphorylase
essential
no
ec
2.5.1.3
synonyms
ipa-26d,ywbK
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.3|Biosynthesis/ acquisition of thiamine]

    • Gene

    Coordinates
    3,930,707 3,931,375

    The protein

    Catalyzed reaction/ biological activity

  • 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H+ --> CO2 + diphosphate + thiamine phosphate (according to UniProt)
  • 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H+ --> CO2 + diphosphate + thiamine phosphate (according to UniProt)
  • 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 4-methyl-5-(2-phosphooxyethyl)-thiazole + H+ --> diphosphate + thiamine phosphate (according to UniProt)

    • Protein family

  • thiamine-phosphate synthase family (single member, according to UniProt)

    • Paralogous protein(s)

  • [protein|CA6AAE8AC5B5EF01A911B035F8782579CD876C08|TenI]

    • Structure

  • [PDB|1G6C] [pubmed|11513589]

    • Expression and Regulation

    Operons

    genes
    [gene|CC55120C2AF455FA2AEAE39E21F57897B2B5BC39|ywbI]-[gene|9B82059498F72A67282DD54892EF02A305E3C674|thiM]-[gene|8FAEB6342E087BEDC16FDF5585B03B90ACFF8D11|thiE]
    description
    [Pubmed|9139923]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9139923], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
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    Biological materials

    Mutant

  • BKE38290 ([gene|8FAEB6342E087BEDC16FDF5585B03B90ACFF8D11|thiE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATTTCCCGAGAAATAC, downstream forward: _UP4_TAATCGTTCATTTTCCTTCA
  • BKK38290 ([gene|8FAEB6342E087BEDC16FDF5585B03B90ACFF8D11|thiE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATTTCCCGAGAAATAC, downstream forward: _UP4_TAATCGTTCATTTTCCTTCA

    • References

    Reviews

  • 19348578

    • Original publications

  • 9139923,11513589