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plsX [Mon Oct 31 2016 11:08:56 GMT+0100 (CET)]
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plsX [Mon Oct 31 2016 11:08:56 GMT+0100 (CET)]

acyl-acyl carrier protein (ACP):phosphate acyltransferase, catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4), coordinates membrane synthesis with cell division
locus
BSU15890
pI
5.00
mw
35.00 kDa
function
biosynthesis of phospholipids
product
acyl-acyl carrier protein (ACP):phosphate acyltransferase
essential
yes
ec
2.3.1.n2
synonyms
ylpD

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|cell division], [SW|biosynthesis of lipids], [SW|essential genes]
  • This gene is a member of the following [SW|regulons]

  • [SW|ComA regulon], [SW|FapR regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    1,662,547 -> 1,663,548

    Phenotypes of a mutant

  • essential [Pubmed|24224907,12682299]
  • depletion results in sporulation deficiency (via [SW|SpoIIR]) [Pubmed|22111992]
  • a ts mutant forms an abberant Z-ring and has a [SW|cell division] defect [Pubmed|24224907]
  • The protein

    Catalyzed reaction/ biological activity

  • catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4)
  • Protein family

  • plsX family (according to Swiss-Prot)
  • Structure

  • [PDB|1VI1] [Pubmed|16021622]
  • [SW|Localization]

  • localizes to the cell division sites, colocalizes with the Z-ring [Pubmed|24224907]
  • uniformly distributed on the membrane of most cells, but occasionally appears as membrane foci as well [Pubmed|26816052]
  • in the presence of daptomycin, PlsX delocalizes from the membrane to the cytoplasm [Pubmed|27791134]
  • [SW|Interactions]

  • [protein|search|PlsX]-[protein|search|PlsY] [Pubmed|19282621]
  • [protein|search|PlsX]-[protein|search|FtsA] [Pubmed|24224907]
  • Expression and Regulation

    Operon

  • ''[protein|search|fapR]-[protein|search|plsX]-[protein|search|fabD]-[protein|search|fabG]-[protein|search|acpA]'' [Pubmed|12737802,19850612]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|16932747]
  • Regulation

  • induced if the cells experience an accumulation of malonyl-CoA ([protein|search|FapR]) [Pubmed|12737802]
  • expressed at high cell density ([protein|search|ComA]) [Pubmed|16091051]
  • induced upon fatty acid biosynthesis inhibition [Pubmed|21383089]
  • Regulatory mechanism

  • [protein|search|ComA]: transcription activation ([protein|search|ComA]) [Pubmed|16091051]
  • [protein|search|FapR]: transcription repression [Pubmed|12737802]
  • Biological materials

    two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in Matsumoto lab [Pubmed|19282621]
  • References

  • 12737802,19282621,17645809,17557823,8759840,16932747,19850612,16091051,21383089,22111992,24224907,26816052,16021622,27791134