nagA

N-acetylglucosamine-6-phosphate deacetylase

Locus: BSU_35010

Isoelectric point: 5.28

Molecular weight: 42.46 kDa

Protein length: 396 aa

Gene length: 1191 bp

Function: N-acetylglucosamine utilization

Product: N-acetylglucosamine-6-phosphate deacetylase

Essential: no

E.C.: 3.5.1.25

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → Utilization of cell wall components
• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of amino sugars
• Metabolism → Amino acid/ nitrogen metabolism → Utilization of nitrogen sources other than amino acids → Utilization of amino sugars

Gene

Coordinates: 3,595,356 3,596,546

Phenotypes of a mutant

• no growth on N-acetylglucosamine PubMed

The protein

Catalyzed reaction/ biological activity

• H₂O + N-acetyl-D-glucosamine 6-phosphate --> acetate + D-glucosamine 6-phosphate (according to UniProt)

Protein family

• Metallo-dependent hydrolases superfamily (according to UniProt)

Kinetic information

• K(M): 1.4 mM PubMed

Structure

• 2VHL PubMed

Expression and Regulation

Operons

• Genes: nagA-nagB-nagR
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • NagR: repression, PubMed, in NagR regulon
  • CcpA: repression, PubMed, in CcpA regulon

  Regulation

  • induced in the presence of N-acetylglucosamine (NagR) PubMed

Biological materials

Mutant

• MGNA-A337 (nagA::erm), available at the NBRP B. subtilis, Japan
• BKE35010 (nagA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGATGATCCGCCTTTCT, downstream forward: _UP4_ATATCCAAGGAGGCTGACCA
• BKK35010 (nagA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGATGATCCGCCTTTCT, downstream forward: _UP4_ATATCCAAGGAGGCTGACCA

References

Reviews

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Original publications

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