chaperonin and co-repressor for HrcA
function
protein folding and re-folding
product
chaperonin, co-repressor for HrcA
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.2|Chaperones/ protein folding][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue][SW|Categories] containing this gene/protein
[SW|chaperones/ protein folding], [SW|heat shock proteins], [SW|essential genes], [SW|phosphoproteins], [SW|most abundant proteins]This gene is a member of the following [SW|regulons]
[SW|HrcA regulon]Gene
Coordinates on the chromosome (coding sequence)
650,234 -> 651,868
Phenotypes of a mutant
essential [Pubmed|12682299]The protein
Catalyzed reaction/ biological activity
acts as co-repressor for [protein|search|HrcA]Protein family
chaperonin (HSP60) family (according to Swiss-Prot)Modification
phosphorylated on Arg-35, Arg-116, and Arg-282 [Pubmed|22517742]phosphorylated on ser/ thr/ tyr [Pubmed|16493705], [Pubmed|17726680]Structure
[PDB|1WE3] ([SW|GroEL]-[SW|GroES] complex from ''Thermus thermophilus'', 65% identity) [Pubmed|15296740][SW|Localization]
cytoplasm (according to Swiss-Prot)recruited to the membrane after ethanol stress [Pubmed|22268681][SW|Interactions]
[protein|search|HrcA]-[SW|GroEL], [SW|GroEL]-[SW|GroES]Expression and Regulation
Operon
''[SW|groES]-[SW|groEL]'' [Pubmed|1350777][SW|Sigma factor]
[protein|search|SigA] [Pubmed|1350776]Regulation
induced by heat shock ([protein|search|HrcA]) [Pubmed|1350777,8576042]Regulatory mechanism
[protein|search|HrcA]: transcription repression [Pubmed|8576042]Additional information
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]Biological materials
Expression vector
for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP1000 available in [SW|Jörg Stülke]'s labfor expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP1002 available in [SW|Jörg Stülke]'s labReferences
9303302,1350777,12082092,15378759,17726680,16493705,9303302,1350776,8576042,7592421,22517742,22268681,24263382,26458230,15296740 
