spoIIIL

component of the SpoIIIA-SpoIIQ trans-envelope complex, required for the activation of SigG

Locus: BSU_24660

Isoelectric point: 10.58

Molecular weight: 9.62 kDa

Protein length: 59 aa

Gene length: 180 bp

Function: activation of SigG

Product: component of the SpoIIIA-SpoIIQ trans-envelope complex

Essential: no

Synonyms: yqzE

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Sporulation → Sporulation/ other

Gene

Coordinates: 2,555,887 2,556,066

Phenotypes of a mutant

• reduced sporulation efficiency (14% compared to wild type) PubMed
• the spoIIIL spoIIIAH double mutant has a severe sporulation defect (0.001%) PubMed

The protein

Localization

• forespore PubMed

Expression and Regulation

Operons

• Genes: comGA-comGB-comGC-comGD-comGE-comGF-comGG-spoIIIL
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigF: sigma factor, PubMed, in SigF regulon

  Regulatory mechanism

  • ComK: activation, PubMed, in ComK regulon

  Regulation

  • expressed early during sporulation in the forespore (SigF) PubMed

  Additional information

  • the comGA-comGB-comGC-comGD-comGE-comGF-comGG-spoIIIL operon requires NusA for expression Reference

• Genes: spoIIIL
  Description: PubMed

  Regulation

  • expressed early during sporulation in the forespore (SigF) PubMed

  Additional information

  • the comGA-comGB-comGC-comGD-comGE-comGF-comGG-spoIIIL operon requires NusA for expression Reference

Biological materials

Mutant

• MGNA-C472 (yqzE::erm), available at the NBRP B. subtilis, Japan
• BKE24660 (spoIIIL::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGTGATCACCTCATCATT, downstream forward: _UP4_TAACCGCAAATAAACGAATA
• BKK24660 (spoIIIL::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGTGATCACCTCATCATT, downstream forward: _UP4_TAACCGCAAATAAACGAATA

References

Reviews

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Original Publications

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