nicK [2019-06-21 10:47:55]

DNA relaxase, similar to transposon protein

Locus: BSU04870

Isoelectric point: 5.13

Molecular weight: 40.78 kDa

Protein length: 352 aa

Gene length: 1059 bp

Function: conjugation of ICE BS1

Product: DNA relaxase, similar to transposon protein

Essential: no

Synonyms: ydcR

Genomic Context

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Categories containing this gene/protein

• Prophages and mobile genetic elements → Mobile genetic elements → ICEBs1

Gene

Coordinates: 534,773 535,831

The protein

Protein family

• plasmid replication initiation factor family (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: xis-ydzL-ydcO-ydcP-ydcQ-nicK-S172-ydcS-ydcT-yddA-conB-conC-conD-conE-yddF-conG-cwlT-yddI-yddJ
  Description: PubMed

  Regulatory mechanism

  • ImmR: repression, PubMed, in ImmR regulon

  Regulation

  • strongly induced in the presence of salt (1.2 M NaCl) PubMed

Biological materials

Mutant

• BKE04870 (nicK::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GCTCATCCATTCGCTTCGCT, downstream forward: _UP4_TAAAACCAAGCTGCTAAACC
• BKK04870 (nicK::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GCTCATCCATTCGCTTCGCT, downstream forward: _UP4_TAAAACCAAGCTGCTAAACC

References

Reviews

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Original Publications

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