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glcK [2019-02-19 20:01:52]
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glcK [2019-02-19 20:01:52]

glucose kinase (D-glucose:ATP)
locus
BSU24850
pI
5.99
mw
33.39 kDa
protein length
321 aa Sequence Blast
gene length
966 bp Sequence Blast
function
phosphorylation of the free glucose moiety resulting from cleavage of di-and oligosaccharides
product
glucose kinase (D-glucose:ATP)
essential
no
ec
2.7.1.2
synonyms
yqgR
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.20|Utilization of trehalose]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.22|Utilization of maltose]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.23|Utilization of starch/ maltodextrin]

    • Gene

    Coordinates
    2,570,606 2,571,571

    The protein

    Catalyzed reaction/ biological activity

  • ATP + D-glucose = ADP + D-glucose 6-phosphate (according to Swiss-Prot)

    • Protein family

  • ROK (nagC/xylR) family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|AF4395D134485290F4AA307B48494FED39E52CD7|XylR]

    • Structure

  • [PDB|2QM1] (from ''Enterococcus faecalis'', 38% identity, 52% similarity)

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|89C51789FBB2EE86771ABD8629FD99748ED5D4EF|yqgP]-[gene|C44F6723A2F1A627A0B9042871A07F5365D6121A|yqgO]-[gene|8E10A8B566BC25CBDDEF1503656A4234FD29ACB8|glcK]-[gene|2B33F2F9C2FB0767B72A29D060037393C88ACEE5|yqgS]
    description
    [pubmed|22383849]

    additional information

  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]∼P binds to the promoter region, but regulation has not been explored [pubmed|25666134]
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    Biological materials

    Mutant

  • GP2 (spc), available in the [SW|Stlke] lab
  • BKE24850 ([gene|8E10A8B566BC25CBDDEF1503656A4234FD29ACB8|glcK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCATATCTCGTCCATTTTTA, downstream forward: _UP4_TAAAATTGTGTAAATGAAAT
  • BKK24850 ([gene|8E10A8B566BC25CBDDEF1503656A4234FD29ACB8|glcK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCATATCTCGTCCATTTTTA, downstream forward: _UP4_TAAAATTGTGTAAATGAAAT

    • References

  • 9620975,15050034,15018644,16707683,7952186,27324299