SubtiBank SubtiBank
glcK [2019-02-19 20:01:52]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

glcK [2019-02-19 20:01:52]

glucose kinase (D-glucose:ATP)
Locus
BSU24850
Isoelectric point
5.99
Molecular weight
33.39 kDa
Protein length
321 aa Sequence Blast
Gene length
966 bp Sequence Blast
Function
phosphorylation of the free glucose moiety resulting from cleavage of di-and oligosaccharides
Product
glucose kinase (D-glucose:ATP)
Essential
no
E.C.
2.7.1.2
Synonyms
yqgR

Genomic Context

      
Loading

Categories containing this gene/protein

Gene

Coordinates
2,570,606 2,571,571

The protein

Catalyzed reaction/ biological activity

  • ATP + D-glucose = ADP + D-glucose 6-phosphate (according to Swiss-Prot)
  • Protein family

  • ROK (nagC/xylR) family (according to Swiss-Prot)
  • Paralogous protein(s)

    Structure

  • 2QM1 (from Enterococcus faecalis, 38% identity, 52% similarity)
  • Localization

  • cell membrane (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    Description

    Additional information

  • PhoP∼P binds to the promoter region, but regulation has not been explored PubMed
  • view in new tab

    Biological materials

    Mutant

  • GP2 (spc), available in the Stlke lab
  • BKE24850 (glcK::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CCATATCTCGTCCATTTTTA, downstream forward: _UP4_TAAAATTGTGTAAATGAAAT
  • BKK24850 (glcK::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CCATATCTCGTCCATTTTTA, downstream forward: _UP4_TAAAATTGTGTAAATGAAAT
  • References

    Loading