bceA [2020-02-06 17:14:38]

primary bacitracin resistance determinant, [SW|ABC transporter] (ATP-binding protein), protects cell wall biosynthetic targets from inhibition by antimicrobial peptides

locus: BSU_30380

pI: 9.29

mw: 28.08 kDa

protein length: 253 aa

gene length: 762 bp

function: protection of cell wall biosynthetic targets from inhibition by antimicrobial peptides

product: [SW|ABC transporter] (ATP-binding protein) for target protection of cell wall synthesis

essential: no

synonyms: ytsC

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.3|Export of peptides]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,111,327 3,112,088

Phenotypes of a mutant

• 85-fold increased sensitivity to bacitracin [Pubmed|26815905]

The protein

Catalyzed reaction/ biological activity

• releaves intermediates of the lipid II cycle from the inhibitory interaction with antimicrobial peptides such as bacitracin [pubmed|31871088]

Protein family

• [SW|ABC transporter] family (according to UniProt)

Paralogous protein(s)

• [protein|FE3A01BEA974C5E32C68DC600C800E5EF101B34F|YknY], [protein|4FE6A51A0D9BBBF574EA2EF18B95B5BFC0A64CCB|YtrE], [protein|8B4267532B9788AEB5EF39E2695C1FDA494C7055|YxdL], [protein|464DD1855E6B3195EE242637612A16C73FF32FC0|PsdA], [protein|0F8048267EC038D8CF673317D969BA27735473A7|YvrO]

[SW|Domains]

• [SW|ABC transporter domain] (aa 4-243) (according to UniProt)

Effectors of protein activity

• the activity of the [protein|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|BceA]-[protein|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|BceB] [SW|ABC transporter] is inhibited by heptaprenyl pyrophosphate which accumulates in a ''[gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB]'' mutant [Pubmed|24806199]

Structure

• [PDB|1L2T] (MJ0796 from '' Methanocaldococcus jannaschii '', 38% identity) [Pubmed|12150914]

[SW|Localization]

• membrane associated (via [protein|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|BceB]) [Pubmed|12890034]

Expression and Regulation

Operons

• genes: [gene|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|bceA]-[gene|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB]
  description: [Pubmed|12890034]

  regulatory mechanism

  • [protein|C239C155F5452C86BF6C78190BABBBB07A63DF87|BceR]: activation, [Pubmed|12890034], in [regulon|C239C155F5452C86BF6C78190BABBBB07A63DF87|BceR regulon]

  regulation

  • induced in the presence of bacitracin, plectasin, mersacidin and actagardine, already at very low levels of bacitracin ([protein|search|BceR]) [Pubmed|12890034,26815905]

Biological materials

Mutant

• MGNA-A167 (ytsC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/167 NBRP B. subtilis, Japan]
• BKE30380 ([gene|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|bceA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTACGCAGTCTCCTTTA, downstream forward: _UP4_CAAGGCGTGTTAGGCGGGGT
• BKK30380 ([gene|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|bceA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTACGCAGTCTCCTTTA, downstream forward: _UP4_CAAGGCGTGTTAGGCGGGGT

labs

• [SW|Susanne Gebhard], Bath UK

References

Reviews

• 27344142,28152228,29404338

Original publications

• 17905982,18394148,12890034,14651641,10092453,14612242,21283517,20606066,21078927,23687272,24806199,25118291,26199330,26364265,12150914,27997719,26815905,31871088