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buk
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buk

butyrate kinase
Locus
BSU_24070
Isoelectric point
5.47
Molecular weight
39.61 kDa
Protein length
363 aa Sequence Blast
Gene length
1092 bp Sequence Blast
Function
utilization of branched-chain keto acids
Product
butyrate kinase
Essential
no
E.C.
2.7.2.7
Synonyms
yqiU,bkd
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of branched-chain amino acids

    • Gene

    Coordinates
    2,501,549 2,502,640

    The protein

    Catalyzed reaction/ biological activity

  • ATP + butanoate --> ADP + butanoyl phosphate (according to UniProt)

    • Protein family

  • acetokinase family (with AckA, according to UniProt)

    • Structure

  • 1X9J (from Thermotoga maritima, 52% identity, 69% similarity)

    • Localization

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    Genes
    ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB
    Description
    PubMed

    Sigma factors

  • SigL: sigma factor, PubMed, in SigL regulon

    • Regulatory mechanism

  • BkdR: activation, PubMed, in BkdR regulon
  • CodY: repression, PubMed, in CodY regulon

    • Regulation

  • induced in the presence of isoleucine or valine (BkdR) PubMed
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    Biological materials

    Mutant

  • MGNA-C378 (yqiU::erm), available at the NBRP B. subtilis, Japan
  • BKE24070 (buk::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTCATAACCTCCAGATCAA, downstream forward: _UP4_TAGACAGACAGGAGTGAGTC
  • BKK24070 (buk::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTCATAACCTCCAGATCAA, downstream forward: _UP4_TAGACAGACAGGAGTGAGTC

    • References

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