single-strand DNA-binding protein, part of the replisome, promoter of RecA DNA repair center assembly, required to internalize and to recombine ssDNA with homologous resident duplex
function
DNA replication, DNA repair/ recombination
product
single-strand DNA-binding protein
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication][category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination][category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue][SW|Categories] containing this gene/protein
[SW|DNA replication], [SW|DNA repair/ recombination], [SW|essential genes], [SW|phosphoproteins]This gene is a member of the following [SW|regulons]
[SW|ComK regulon], [SW|stringent response]Gene
Coordinates on the chromosome (coding sequence)
4,198,886 -> 4,199,404
Phenotypes of a mutant
essential [Pubmed|12682299]The protein
Catalyzed reaction/ biological activity
SsbA inhibits ssDNA phosphorylase activity of [protein|search|PnpA] [Pubmed|21859751]SsbA co-assembles onto [protein|search|SsbB]-coated ssDNA [Pubmed|22373918]important for [protein|search|RecA] DNA repair center assembly by recruiting [protein|search|RecO] and [protein|search|RecR] to the [SW|replisome] [Pubmed|24891441]RecA-ATP in concert with [protein|search|DprA] and [protein|search|SsbA] catalyzes DNA strand exchange, with [protein|search|SsbB] as an accessory factor [Pubmed|25138221]Paralogous protein(s)
[protein|search|SsbB]Modification
phosphorylated on Arg-76 [Pubmed|22517742]phosphorylated on Tyr-82 by [protein|search|PtkA], dephosphorylated by [protein|search|PtpZ] [Pubmed|16549871]Effectors of protein activity
Phosphorylation of Ssb increases binding to single-stranded DNA in vitro almost 200-fold [Pubmed|16549871]Structure
[PDB|3VDY] (SsbB in complex with ssDNA, 63% identity, 87% similarity) [Pubmed|22373918][SW|Localization]
forms foci [Pubmed|21958350]Nucleoid (Mid-cell) [Pubmed|16479537][SW|Interactions]
forms tetramers [Pubmed|22373918]part of the [SW|replisome]: [protein|search|PolC]-[protein|search|HolA]-[protein|search|HolB]-[protein|search|DnaX]-[protein|search|DnaN]-[protein|search|DnaG]-[protein|search|DnaC]-[protein|search|DnaI]-[protein|search|DnaD]-[protein|search|SsbA]-[protein|search|DnaE]-[protein|search|PriA]-[protein|search|DnaB] [Pubmed|20122408][protein|search|SsbA]-[protein|search|SbcC] [Pubmed|21170359][protein|search|SsbA]-[protein|search|RecG] [Pubmed|21170359][protein|search|SsbA]-[protein|search|RecJ] [Pubmed|21170359][protein|search|SsbA]-[protein|search|YrvN] [Pubmed|21170359][protein|search|SsbA] (bound to ssDNA) -[protein|search|RecO], this helps to dislodge [protein|search|SsbA] and [protein|search|SsbB] from ssDNA [Pubmed|21170359,22373918][protein|search|SsbA]-[protein|search|YqiB] [Pubmed|21170359][protein|search|SsbA]-[protein|search|Ung] [Pubmed|21170359][protein|search|SsbA]-[protein|search|YpbB] [Pubmed|21170359][protein|search|SsbA]-[SW|RecD2] [Pubmed|24443534,21170359][protein|search|SsbA]-[protein|search|DnaN] [Pubmed|21958350][protein|search|SsbA] (bound to ssDNA) -[protein|search|RecO], this helps to dislodge [protein|search|SsbA] and [protein|search|SsbB] from ssDNA [Pubmed|21170359,22373918][protein|search|SsbA]-[protein|search|YocI] according to [Pubmed|22933559][protein|search|SsbA]-[protein|search|RecQ] according to [Pubmed|22933559]Expression and Regulation
Operon
''[protein|search|yyaF]-[protein|search|rpsF]-[protein|search|ssbA]-[protein|search|rpsR]'' [Pubmed|14762004]''[protein|search|rpsF]-[protein|search|ssbA]-[protein|search|rpsR]'' [Pubmed|24310371,22383849][SW|Sigma factor]
''[protein|search|rpsF]'': [protein|search|SigA] [Pubmed|24310371]Regulation
[protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165][protein|search|ComK]: transcription activation [Pubmed|11948146]Biological materials
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s labReferences
Reviews
Original publications
17853894,19730681,11948146,16479537,11948165,16549871,20122408,14762004,20581116,22054219,22373918,22517742,21859751,21170359,21958350,23268446,23536821,23779106,22383849,24310371,24443534,24891441,25138221,26707201 
