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ssbA [2020-04-28 18:30:31]
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ssbA [2020-04-28 18:30:31]

single-strand DNA-binding protein, part of the [SW|replisome], promoter of [protein|search|RecA ]DNA repair center assembly, required to internalize and to recombine ssDNA with homologous resident duplex
locus
BSU_40900
pI
4.82
mw
18.60 kDa
protein length
172 aa Sequence Blast
gene length
519 bp Sequence Blast
function
[category|SW 3.1.1|DNA replication], [category|SW 3.1.5|DNA repair/ recombination]
product
single-strand DNA-binding protein
essential
yes
synonyms
ssb
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue]

    • Gene

    Coordinates
    4,198,886 4,199,404

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • SsbA inhibits ssDNA phosphorylase activity of [protein|64C6D783FF3F41C81B216F798A1DC8071345B1ED|PnpA] [Pubmed|21859751]
  • SsbA co-assembles onto [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|SsbB]-coated ssDNA [Pubmed|22373918]
  • important for [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] DNA repair center assembly by recruiting [protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|RecO] and [protein|BEBAF1EB53EBB3E7558BB5FE73C418B50000FA29|RecR] to the [SW|replisome] [Pubmed|24891441]
  • [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA]-ATP in concert with [protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|DprA] and [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|SsbA] catalyzes DNA strand exchange, with [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|SsbB] as an accessory factor [Pubmed|25138221]
  • inhibits stable [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] nucleation and polymerization on ssDNA [pubmed|28911099]
  • the [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|SsbA]-[protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|DprA] mediator loads [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] onto any fragmented linear SPP1 ssDNA[pubmed|31876108]

    • Paralogous protein(s)

  • [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|SsbB]

    • [SW|Domains]

  • SSB domain (aa 1-104) (according to UniProt)

    • Modification

  • phosphorylated on Arg-76 [Pubmed|22517742]
  • phosphorylated on Tyr-82 by [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|PtkA], dephosphorylated by [protein|CF73D86DB024575BE8F043A4C3996458F4262E46|PtpZ] [Pubmed|16549871]

    • Effectors of protein activity

  • Phosphorylation of Ssb increases binding to single-stranded DNA in vitro almost 200-fold [Pubmed|16549871]

    • Structure

  • [PDB|6BHX] (complex with ssDNA) [Pubmed|30472092]
  • [PDB|6BHW] [Pubmed|30472092]

    • [SW|Localization]

  • forms foci [Pubmed|21958350]
  • Nucleoid (Mid-cell) [Pubmed|16479537]

    • Expression and Regulation

    Operons

    genes
    [gene|BD162A17E5F3F9AB49E478CE72D0FE92D82E6673|yyzM]-[gene|FEE1952DFA3D6A5E2DC108B3B203B526642E2C68|yyaE]-[gene|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF]-[gene|9D25B8F4579C41E77AE9CDD18CAD6DA37F3DD0A2|rpsF]-[gene|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]-[gene|C0B926F1134A7A3FEEC886F9AF67F694C8E0C089|rpsR]
    description
    [Pubmed|22383849]
    view in new tab

    genes
    [gene|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF]-[gene|9D25B8F4579C41E77AE9CDD18CAD6DA37F3DD0A2|rpsF]-[gene|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]-[gene|C0B926F1134A7A3FEEC886F9AF67F694C8E0C089|rpsR]
    description
    [Pubmed|14762004]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|24310371], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]
  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

    • regulation

  • [protein|search|ComK]: transcription activation [Pubmed|11948146]
    • view in new tab

    genes
    [gene|9D25B8F4579C41E77AE9CDD18CAD6DA37F3DD0A2|rpsF]-[gene|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]-[gene|C0B926F1134A7A3FEEC886F9AF67F694C8E0C089|rpsR]
    description
    [Pubmed|24310371,22383849]

    regulation

  • [protein|search|ComK]: transcription activation [Pubmed|11948146]
    • view in new tab

    Biological materials

    two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab

    • References

    Reviews

  • 22976189,22933559,31950915

    • Original publications

  • 17853894,19730681,11948146,16479537,11948165,16549871,20122408,14762004,20581116,22054219,22373918,22517742,21859751,21170359,21958350,23268446,23536821,23779106,22383849,24310371,24443534,24891441,25138221,26707201,26786319,28575448,28878042,29059392,29059328,28911099,30472092,31876108