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tkmA [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]
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tkmA [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

transmembrane modulator of PtkA activity, activates PtkA autophosphorylation and substrate phosphorylation
locus
BSU36260
pI
4.00
mw
26.00 kDa
function
control of protein tyrosine phosphorylation
product
modulator of PtkA activity
essential
no
synonyms
ywqC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.4|Regulation]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • [SW|Categories] containing this gene/protein

  • [SW|biofilm formation], [SW|protein modification], [SW|membrane proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|AbrB regulon], [SW|DegU regulon], [SW|Spo0A regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    3,732,525 -> 3,733,271

    Phenotypes of a mutant

  • the mutant exhibits a defect in [SW|biofilm formation], pronounced on LBGM medium, weak of MSgg medium [Pubmed|26283769,20815827], this can be compensated for by overexpression of ''[protein|search|epsA]'' or by mutations that reduce the expression of [protein|search|SinR] [Pubmed|26283769]

    • The protein

    Catalyzed reaction/ biological activity

  • transmembrane activation of [protein|search|PtkA] protein tyrosine kinase activity

    • Protein family

  • cpsC/capA family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|search|EpsA]

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot)

    • [SW|Interactions]

  • [protein|search|PtkA]-[protein|search|TkmA] [Pubmed|12970183]
  • [protein|search|FatR]-[protein|search|TkmA] [Pubmed|23939619]

    • Expression and Regulation

    Operon

  • ''[protein|search|tkmA]-[protein|search|ptkA]-[protein|search|ptpZ]-[protein|search|ugd]'' [Pubmed|20815827,12970183]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|20815827]

    • Regulatory mechanism

  • [protein|search|AbrB]: transcription repression [Pubmed|20817675]
  • [SW|Spo0A]: transcription activation [Pubmed|26283769]
  • [protein|search|DegU]-P: transcription activation [Pubmed|26283769]

    • Biological materials

    Mutant

  • GP1566 (spc) [Pubmed|24493247], available in [SW| Jörg Stülke]'s lab
  • GP1567 ''[protein|search|epsA]''::aphA3 ''[protein|search|tkmA]''::spc [Pubmed|24493247], available in [SW| Jörg Stülke]'s lab

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW| Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP1620 ''tkmA''-FLAG 3x spc (based on [SW|pGP1331]) available in [SW| Jörg Stülke]'s lab

    • References

  • 12970183,20815827,20817675,23939619,24493247,26283769,27725816