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cwlT [2018-11-07 16:52:28]
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cwlT [2018-11-07 16:52:28]

cell wall hydrolase, required for conjugation of ICEBs1, part of the type IV secretion system for DNA transfer , C-terminal domain hydrolyzes bond between D-Glu and m-DAP
Locus
BSU04970
Isoelectric point
8.63
Molecular weight
36.39 kDa
Protein length
329 aa Sequence Blast
Gene length
987 bp Sequence Blast
Function
conjugative transfer of ICEBs1
Product
cell wall hydrolase
Synonyms
yddH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → Autolysis
  • Prophages and mobile genetic elements → Mobile genetic elements → ICEBs1

    • Gene

    Coordinates
    544,022 → 545,011

    The protein

    Catalyzed reaction/ biological activity

  • hydrolysis of peptidoglycan (linkage between N-acetylmuramic acid and N-acetylglucosamine and bond between D-γ-glutamate and meso-diaminopimelic acid) PubMed
  • degradation of gamma-polyglutamic acid PubMed

    • Protein family

  • nlpC/p60 family (according to Swiss-Prot)

    • Domains

  • N-terminal domain is an N-acetylmuramidase that cleaves the linkage between N-acetylmuramic acid and N-acetylglucosamine PubMed
  • C-terminal D,L-endopeptidase domain (NlpC/P60 domain) PubMed, cleaves the bond between D-γ-glutamate and meso-diaminopimelic acid PubMed

    • Effectors of protein activity

  • both enzymatic activities are inhibited by interaction with IseA PubMed

    • Structure

  • 4FDY (from Staphylococcus aureus, 42% identity) PubMed

    • Localization

  • secreted (with signal peptide) PubMed
  • may be a lipoprotein, but the lipid anchor is not required for function PubMed

    • Expression and Regulation

    Operons

    Genes
    xis-ydzL-ydcO-ydcP-ydcQ-nicK-S172-ydcS-ydcT-yddA-conB-conC-conD-conE-yddF-conG-cwlT-yddI-yddJ
    Description
    PubMed

    Regulatory mechanism

  • ImmR: repression, PubMed, in ImmR regulon

    • Regulation

  • strongly induced in the presence of salt (1.2 M NaCl) PubMed
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    Biological materials

    Mutant

  • BKE04970 (ΔcwlT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TATCATCTTGTTCTTTCATC, downstream forward: _UP4_ATTAAATAACTAGGAGTGAA
  • BKK04970 (ΔcwlT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TATCATCTTGTTCTTTCATC, downstream forward: _UP4_ATTAAATAACTAGGAGTGAA

    • References

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