yhcY

two-component sensor kinase

locus: BSU_09320

pI: 6.64

mw: 41.83 kDa

protein length: 379 aa

gene length: 1140 bp

function: unknown

product: two-component sensor kinase

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

Gene

Coordinates: 1,008,668 1,009,807

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of [protein|DD799ED3EB79457C27ED30C7B4DBBC2C8F962191|YhcZ] in a Asp residue
• ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

[SW|Domains]

• [SW|Histidine kinase domain] (aa 185-373) (according to UniProt)

Modification

• autophosphorylation on a His residue

[SW|Localization]

• cytoplasm (Homogeneous) [Pubmed|16479537]

Expression and Regulation

Operons

• genes: [gene|8D570AEE5D78A9A3CDBB1F457630BAF5DB59BCFD|yhcY]-[gene|DD799ED3EB79457C27ED30C7B4DBBC2C8F962191|yhcZ]-[gene|345EC248C0ADF48B0C25B175241AAE982E0E9B78|yhdA]
  description: [Pubmed|16816187]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16816187], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|LiaR]: activation, [Pubmed|16816187], in [regulon|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|LiaR regulon]

Biological materials

Mutant

• MGNA-A729 (yhcY::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/729 NBRP B. subtilis, Japan]
• BKE09320 ([gene|8D570AEE5D78A9A3CDBB1F457630BAF5DB59BCFD|yhcY]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTTCTCACTCCGGCAT, downstream forward: _UP4_AAAAGCCGAAAAGGAGGGGC
• BKK09320 ([gene|8D570AEE5D78A9A3CDBB1F457630BAF5DB59BCFD|yhcY]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTTCTCACTCCGGCAT, downstream forward: _UP4_AAAAGCCGAAAAGGAGGGGC

References

• 10094672,20639339,16816187,16479537,29785949