SubtiBank SubtiBank
clpE [2019-06-04 17:25:13]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

clpE [2019-06-04 17:25:13]

AAA unfoldase, ATPase subunit of the ClpE-ClpP protease (class III stress gene)
Locus
BSU13700
Isoelectric point
5.14
Molecular weight
77.72 kDa
Protein length
699 aa Sequence Blast
Gene length
2100 bp Sequence Blast
Function
protein degradation
Product
AAA unfoldase, ATPase subunit of the ClpE-ClpP protease
Essential
no
Synonyms
ykvH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
Loading

Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Proteolysis → Additional proteins involved in proteolysis
  • Lifestyles → Coping with stress → Heat shock proteins

    • Gene

    Coordinates
    1,435,628 1,437,727

    The protein

    Catalyzed reaction/ biological activity

  • ATPase/chaperone

    • Protein family

  • ClpA/ClpB family (with ClpC, according to UniProt)

    • Paralogous protein(s)

  • ClpC

    • Domains

  • AAA-ATPase PFAM

    • Structure

  • 6EM8 (ClpC from Staphylococcus aureus, 54% identity) PubMed

    • Localization

  • localization in cytoplasmic polar clusters, excluded from the nucleoid, colocalization with ClpP Pubmed
  • forms foci coincident with nucleoid edges, usually near cell poles PubMed
  • [[File:ClpE.jpg ]]

    • Expression and Regulation

    Operons

    Genes
    clpE
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • CtsR: repression, PubMed, in CtsR regulon

    • Regulation

  • induced by heat (CtsR) PubMed
    • view in new tab

    Biological materials

    Mutant

  • clpE::spec available from the Hamoen] Lab
  • BKE13700 (clpE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTGCCAAAACCTCCTTA, downstream forward: _UP4_TAACAATCAGCGGTTTCCTT
  • BKK13700 (clpE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTGCCAAAACCTCCTTA, downstream forward: _UP4_TAACAATCAGCGGTTTCCTT

    • GFP fusion

  • C-terminal YFP and CFP fusions (single copy) available from the Hamoen] Lab

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

    • FLAG-tag construct

  • GP1799 (spc, based on pGP1331), available in Jörg Stülke's lab

    • Antibody

  • available in Ulf Gerth's and Jörg Stülke's labs

    • References

    Reviews

    Loading

    Original Publications

    Loading

    Labs working on this gene/protein

  • Leendert Hamoen, Newcastle University, UK homepage