clpE [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

AAA unfoldase, ATPase subunit of the ClpE-ClpP protease (class III stress gene)

locus: BSU13700

pI: 5.00

mw: 77.00 kDa

function: protein degradation

product: AAA unfoldase, ATPase subunit of the ClpE-ClpP protease

essential: no

synonyms: ykvH

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]

[SW|Categories] containing this gene/protein

• [SW|proteolysis], [SW|heat shock proteins]

This gene is a member of the following [SW|regulons]

• [SW|CtsR regulon]

Gene

Coordinates on the chromosome (coding sequence): 1,435,628 -> 1,437,727

The protein

Catalyzed reaction/ biological activity

• ATPase/chaperone

Protein family

• AAA -type ATPase (IPR013093) [http://www.ebi.ac.uk/interpro/IEntry?ac=IPR013093 InterPro] (PF07724) [http://pfam.sanger.ac.uk/family?acc=PF07724 PFAM]

Paralogous protein(s)

• [protein|search|ClpC], [protein|search|ClpX]

[SW|Domains]

• AAA-ATPase [http://pfam.sanger.ac.uk/family?acc=PF07724 PFAM]

[SW|Localization]

• localization in cytoplasmic polar clusters, excluded from the nucleoid, colocalization with [protein|search|ClpP] [http://www.ncbi.nlm.nih.gov/pubmed/18786145 Pubmed]
• forms foci coincident with nucleoid edges, usually near cell poles [Pubmed|18689473]
• [[File:ClpE.jpg‎ ]]

[SW|Interactions]

• forms hexamers [Pubmed|26639779]
• [protein|search|ClpE]-[protein|search|ClpP]

Expression and Regulation

Operon

• ''clpE'' [Pubmed|10320580]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|10320580]

Regulation

• induced by heat ([protein|search|CtsR]) [Pubmed|10320580,9987115]

Regulatory mechanism

• [protein|search|CtsR]: transcription repression [Pubmed|10320580,9987115,11179229,16163393,17380125]

Biological materials

Mutant

• ''clpE::spec'' available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen]] Lab

GFP fusion

• C-terminal YFP and CFP fusions (single copy) available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen]] Lab

Antibody

• available in [SW|Ulf Gerth]'s and [SW|Jörg Stülke]'s labs

Labs working on this gene/protein

• [SW|Leendert Hamoen], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen homepage]

References

• 17302811,26639779